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首页> 外文期刊>Canadian journal of surgery: Journal canadien de chirurgie >Melatonin ameliorates liver fibrosis induced by bile-duct ligation in rats.
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Melatonin ameliorates liver fibrosis induced by bile-duct ligation in rats.

机译：褪黑素改善了大鼠胆管结扎引起的肝纤维化。

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BACKGROUND: Hepatic stellate cells, the main mediators in the pathogenesis of fibrosis, are triggered by free radicals and produce collagen. Melatonin is a powerful physiologic scavenger of hydroxyl radicals. It is also involved in the inhibitory regulation of the collagen content in tissue. There is no effective treatment available for liver fibrosis. Our objective was to evaluate the effects of melatonin on liver fibrosis induced by bile-duct ligation (BDL) in rats. METHODS: We divided male Wistar rats (n = 32) into 4 groups. Two groups received BDL and 2 groups received sham operations. One of the BDL groups and one of the sham groups were administered melatonin (100 mg/kg/day via intraperitoneal injection), and the controls were given vehicle only. After 1 month, we biochemically evaluated the changes in hepatic fibrosis by measuring tissue collagen levels and histopathologic examination. We evaluated the levels of malondialdehyde (MDA), glutathione (GSH), luminal and lucigenin in tissue homogenates, and we studied proinflammatory cytokines in serum using commercially available kits. RESULTS: Bile-duct ligation caused hepatic fibrotic changes, whereas melatonin suppressed these changes in 5 of 8 rats (p < 0.001). Bile-duct ligation resulted in increased collagen, MDA, luminal and lucigenin levels and decreased GSH levels, whereas melatonin reversed these effects. CONCLUSION: We found that melatonin functions as an effective fibrosuppressant and antioxidant, and the results suggest that it can be used as a therapeutic option.
机译：背景：肝星状细胞是纤维化发病机理中的主要介质，被自由基触发并产生胶原蛋白。褪黑激素是羟自由基的强大生理清除剂。它也参与组织中胶原蛋白含量的抑制性调节。没有有效的肝纤维化治疗方法。我们的目的是评估褪黑激素对大鼠胆汁结扎（BDL）所致肝纤维化的影响。方法：我们将雄性Wistar大鼠（n = 32）分为4组。 2组接受BDL，2组接受假手术。给BDL组之一和假组之一给予褪黑激素（通过腹膜内注射每天100 mg / kg /天），对照组仅给予媒介物。 1个月后，我们通过测量组织胶原蛋白水平和组织病理学检查对肝纤维化的变化进行了生化评估。我们评估了组织匀浆中丙二醛（MDA），谷胱甘肽（GSH），管腔和发光素的水平，并使用市售试剂盒研究了血清中的促炎细胞因子。结果：胆管结扎导致肝纤维化改变，而褪黑激素抑制了8只大鼠中的5只的这些变化（p <0.001）。胆管结扎导致胶原蛋白，MDA，管腔和光泽蛋白水平升高，而谷胱甘肽水平降低，而褪黑激素则逆转了这些作用。结论：我们发现褪黑激素可作为一种有效的纤维抑制剂和抗氧化剂，结果表明它可作为一种治疗选择。

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