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首页> 外文期刊>The American Journal of the Medical Sciences >Dysfunction of splenic macrophages in cirrhotic patients with hypersplenism and HBV infection.
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Dysfunction of splenic macrophages in cirrhotic patients with hypersplenism and HBV infection.

机译：肝硬化合并脾功能亢进和乙肝病毒感染患者的脾脏巨噬细胞功能障碍。

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BACKGROUND:: Hepatitis B virus (HBV) reinfection is a difficult problem to manage after liver transplantation in patients with cirrhosis. This study was designed to investigate the activation type of splenic macrophages in cirrhotic patients with hypersplenism and HBV infection to assess the immune function of splenic macrophages. METHODS:: Fourteen cirrhotic patients with hypersplenism and HBV infection and 6 controls were enrolled in the study. Serum lipopolysaccharide (LPS) was detected with a limulus assay. The differential expression of cytokines by splenic tissue and splenic macrophages between the cirrhosis and control groups was compared with cytokine arrays. Furthermore, splenic macrophages were cultured and stimulated with LPS, after which tumor necrosis factor (TNF)-alpha and interleukin (IL)-12 levels in the supernatant were determined. RESULTS:: In cirrhotic patients, serum LPS levels increased significantly. Interferon-gamma, TNF-beta, and transforming growth factor-beta upregulated, whereas IL-4 and IL-13 levels did not change in splenic tissue. TNF-alpha upregulated significantly, whereas IL-4 and IL-5 levels had no significant changes in splenic macrophages. The IL-12 levels in culture media of splenic macrophages from cirrhotic patients were significantly lower than in controls after LPS stimulation. CONCLUSION:: Splenic macrophages may be activated via incomplete M1 activation in cirrhotic patients with hypersplenism and HBV infection, and the immune function of splenic macrophages is impaired.
机译：背景：乙肝病毒（HBV）的再感染是肝硬化患者肝移植后难以处理的问题。本研究旨在调查肝硬化性脾功能亢进和HBV感染患者脾脏巨噬细胞的激活类型，以评估脾脏巨噬细胞的免疫功能。方法：本研究招募了14例肝硬化伴脾功能亢进和HBV感染的患者和6名对照。用测定法检测血清脂多糖（LPS）。比较了肝硬化组和对照组之间脾脏组织和脾脏巨噬细胞的细胞因子表达差异。此外，将脾巨噬细胞培养并用LPS刺激，然后测定上清液中的肿瘤坏死因子（TNF）-α和白介素（IL）-12的水平。结果：在肝硬化患者中，血清脂多糖水平显着升高。干扰素-γ，TNF-β和转化生长因子-β上调，而脾脏组织中IL-4和IL-13的水平没有变化。 TNF-α明显上调，而脾脏巨噬细胞中IL-4和IL-5水平无明显变化。肝硬化患者脾脏巨噬细胞培养基中的IL-12水平明显低于LPS刺激后的对照组。结论：脾功能亢进和HBV感染的肝硬化患者可能通过不完全的M1活化来激活脾脏巨噬细胞，并且脾脏巨噬细胞的免疫功能受损。

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