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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Comparison of five methods of malaria detection in the outpatient setting.
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Comparison of five methods of malaria detection in the outpatient setting.

机译：门诊环境中五种疟疾检测方法的比较。

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In eastern Africa where 90% of the malaria is due to Plasmodium falciparum, the accuracy of malaria diagnosis at the outpatient level is becoming increasingly important due to problems of drug resistance and use of alternative, costly antimalarial drugs. The quantitative buffy coat (QBC) technique, acridine orange staining with an interference filter system, and the ParaSight-F test have been introduced as alternative methods to conventional microscopy for the diagnosis of malaria. Two hundred thirteen outpatients were tested using these alternative methods and conventional microscopy by five experienced technologists; two were randomly allocated to read the results of each test. Paired results showed the highest level of agreement with the ParaSight-F test (99%), followed by Field stain (92%). The results of the QBC technique showed the least agreement (73%). Using conventional microscopy as the reference standard, the ParaSight-F test had a sensitivity range of 90-92% and a specificity of 99%, staining with acridine orange had a sensitivity range of 77-96% and a specificity range of 81-98% and the QBC technique had a sensitivity range of 88-98% and a specificity range of 58-90%. All microscopic tests showed lower sensitivities (as low as 20% using staining with acridine orange) in detecting low parasitemias (< or = 320/microl) than the ParaSight-F test (70%). Due to the high cost of the ParaSight-F test, Field-stained blood films remain the most appropriate method for diagnosis of P. falciparum in eastern Africa. The ParaSight-F test may be used in situations where no trained microscopists are available, or where malaria is strongly suspected and the results of microscopy are negative.
机译：在东部非洲，其中90％的疟疾是由恶性疟原虫引起的，由于耐药性问题以及使用其他昂贵的抗疟药的问题，门诊水平的疟疾诊断准确性越来越重要。已引入定量血沉棕黄层（QBC）技术，采用干涉滤光片系统的a啶橙染色和ParaSight-F测试，作为常规显微镜的替代方法，可用于诊断疟疾。由五位经验丰富的技术人员使用这些替代方法和常规显微镜对213位门诊病人进行了测试;随机分配两个，以读取每个测试的结果。配对结果显示，与ParaSight-F测试的一致性最高（99％），其次是场染色（92％）。 QBC技术的结果显示一致性最低（73％）。使用常规显微镜作为参考标准，ParaSight-F测试的灵敏度范围为90-92％，特异性为99％，with啶橙染色的灵敏度范围为77-96％，特异性范围为81-98 ％和QBC技术具有88-98％的灵敏度范围和58-90％的特异性范围。与ParaSight-F测试（70％）相比，所有显微镜测试均显示在检测低寄生虫病（<或= 320 / microl）时灵敏度较低（使用a啶橙染色可低至20％）。由于ParaSight-F测试的高昂费用，现场染色的血膜仍然是诊断东非恶性疟原虫的最合适方法。在没有训练有素的显微镜医师的情况下，或者在强烈怀疑疟疾且显微镜检查结果为阴性的情况下，可以使用ParaSight-F测试。

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