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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Evaluation of recombinant Plasmodium knowlesi merozoite surface protein-133for detection of human malaria
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Evaluation of recombinant Plasmodium knowlesi merozoite surface protein-133for detection of human malaria

机译：重组诺氏疟原虫裂殖子表面蛋白-133在检测人类疟疾中的评价

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摘要

Plasmodium knowlesi is now known as the fifth Plasmodium species that can cause human malaria. The Plasmodium merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-133 has been shown to be immunogenic and its T cell epitopes could mediate cellular immune protection. However, limited studies have focused on P. knowlesi MSP-133. In this study, an approximately 28-kDa recombinant P. knowlesi MSP-133 (pkMSP-133) was expressed by using an Escherichia coli system. The purified pkMSP-133 reacted with serum samples of patients infected with P. knowlesi (31 of 31, 100%) and non-P. knowlesi malaria (27 of 28, 96.43%) by Western blotting. The pkMSP-1 33 also reacted with P. knowlesi (25 of 31, 80.65%) and non-P. knowlesi malaria sera (20 of 28, 71.43%) in an enzyme-linked immunosorbent assay (ELISA). Most of the non-malarial infection (49 of 52 in by Western blotting and 46 of 52 in the ELISA) and healthy donor serum samples (65 of 65 by Western blotting and ELISA) did not react with recombinant pkMSP-133.
机译：现在已知诺氏疟原虫是第五类可引起人类疟疾的疟原虫。据报道，疟原虫裂殖子表面蛋白（MSP）是接种疫苗和诊断疟疾的潜在目标。 MSP-133已被证明具有免疫原性，其T细胞表位可以介导细胞免疫保护。但是，有限的研究集中在诺氏疟原虫MSP-133上。在这项研究中，通过使用大肠杆菌系统表达了约28 kDa的重组诺氏疟原虫MSP-133（pkMSP-133）。纯化的pkMSP-133与感染诺氏假单胞菌（31个，占31％，100％）的患者的血清样品反应。 Western印迹检测Knowlesi疟疾（28/27，96.43％）。 pkMSP-1 33也与诺氏假单胞菌（25分，占31，80.65％）和非P.酶联免疫吸附测定（ELISA）中的Knowlesi疟疾血清（28 of 20，71.43％）。大多数非疟疾感染（通过Western印迹检测的49例中占49例，在ELISA中52检测的46例中）和健康的供体血清样品（通过Western印迹和ELISA的65例中有65例）未与重组pkMSP-133反应。

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