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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.
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Type-3 dengue viruses responsible for the dengue epidemic in Malaysia during 1993-1994.

机译:1993年至1994年期间导致马来西亚登革热流行的3型登革热病毒。

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摘要

To characterize the dengue epidemic that recently occurred in Malaysia, we sequenced cDNAs from nine 1993-1994 dengue virus type-3 (DEN-3) isolates in Malaysia (DEN-3 was the most common type in Malaysia during this period). Nucleic acid sequences (720 nucleotides in length) from the nine isolates, encompassing the precursor of membrane protein (preM) and membrane (M) protein genes and part of the envelope (E) protein gene were aligned with various reference DEN-3 sequences to generate a neighbor-joining phylogenetic tree. According to the constructed tree, the nine Malaysian isolates were grouped into subtype II, which comprises Thai isolates from 1962 to 1987. Five earlier DEN-3 virus Malaysian isolates from 1974 to 1981 belonged to subtype I. The present data indicate that the recent dengue epidemic in Malaysia was due to the introduction of DEN-3 viruses previously endemic to Thailand.
机译:为了表征最近在马来西亚发生的登革热流行,我们对来自马来西亚的9种1993-1994年登革热病毒3型(DEN-3)分离株进行了cDNA测序(在此期间,DEN-3是马来西亚最常见的一种)。来自九个分离株的核酸序列(长度为720个核苷酸),包括膜蛋白(preM)和膜(M)蛋白基因的前体以及部分包膜(E)蛋白基因,与各种参考DEN-3序列比对生成一个相邻的系统发育树。根据构建的树,将9个马来西亚分离株分为II型,其中包括1962年至1987年的泰国分离株。1974年至1981年有5个较早的DEN-3病毒马来西亚分离株属于I型。目前的数据表明,最近的登革热马来西亚的流行是由于引入了泰国特有的DEN-3病毒。

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