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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Venezuelan equine encephalitis and Oropouche virus infections among Peruvian army troops in the Amazon region of Peru.
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Venezuelan equine encephalitis and Oropouche virus infections among Peruvian army troops in the Amazon region of Peru.

机译：秘鲁亚马孙地区秘鲁军队中的委内瑞拉马脑炎和Oropouche病毒感染。

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An outbreak of a febrile illness characterized by headache, ocular pain, myalgia, and arthralgia occurred during June 1994 among Peruvian army troops in Northern Peru. On June 14-16, 1994, clinical data and blood samples were obtained from eight soldiers with a febrile illness, and from 26 others who had a history of febrile illness during the past three months. A follow-up blood sample was obtained 107 days later from four of the febrile and seven of the afebrile soldiers. Serum samples were tested for dengue (DEN), Oropouche (ORO), and Venezuelan equine encephalitis (VEE) IgM and IgG antibodies by an enzyme-linked immunosorbent assay (ELISA). Virus isolation was performed by inoculation of newborn mice and Vero cell cultures. Viral isolates were identified by immunofluorescence, ELISA, and nucleotide sequencing. A VEE virus infection was confirmed in three of the eight febrile soldiers, two by virus isolation, and one by serology. Antigenic analysis indicated that one of the virus isolates was similar to VEE subtype I, variety ID, viruses previously isolated in Colombia and Venezuela. Nucleotide sequence data showed that both viral isolates were identical to one another and closely related to VEE ID viruses previously isolated in Peru, Colombia, and Venezuela. Serologic results showed that two of 26 afebrile soldiers had IgM antibody to VEE and four had IgG antibody to VEE; two febrile soldiers had IgG antibody in their first serum samples. Oropouche-specific IgM antibody was detected in one of the eight febrile and five of the afebrile soldiers, and 18 of the 34 soldiers had low titers of ORO IgG antibody titers, which did not meet the diagnostic criteria for confirmed cases. All soldiers were negative for DEN IgM antibody, and 10 had flavivirus IgG antibody that reacted with DEN antigens. These data indicated that VEE ID virus was one of the causes of illness among Peruvians soldiers and that this was the first association of this VEE subtype with human disease in Peru.
机译：1994年6月，秘鲁北部的秘鲁军队中爆发了以头痛，眼痛，肌痛和关节痛为特征的高热病暴发。 1994年6月14日至16日，从八名有发热病的士兵以及过去三个月内有发热病史的其他26名士兵那里获得了临床数据和血液样本。 107天后，从四名发热的士兵和七名发热的士兵那里获得了随访血样。通过酶联免疫吸附测定（ELISA）检测血清样本的登革热（DEN），Oropouche（ORO）和委内瑞拉马脑炎（VEE）IgM和IgG抗体。通过接种新生小鼠和Vero细胞培养物进行病毒分离。通过免疫荧光，ELISA和核苷酸测序鉴定病毒分离株。在八名发热士兵中，有三名确认了VEE病毒感染，其中两名通过病毒分离，另一名通过血清学证实。抗原分析表明，其中一种病毒分离物类似于先前在哥伦比亚和委内瑞拉分离出的VEE亚型I（品种ID）。核苷酸序列数据显示，这两种病毒分离株彼此相同，并且与先前在秘鲁，哥伦比亚和委内瑞拉分离出的VEE ID病毒密切相关。血清学结果显示，在26名发热士兵中，有2人具有针对VEE的IgM抗体，另外4人具有针对VEE的IgG抗体。两名发热士兵的第一份血清样品中含有IgG抗体。在八名发热的士兵和五名发热的士兵之一中检测到了Oropouche特异性IgM抗体，在34名士兵中，有18名的ORO IgG抗体滴度很低，不符合确诊病例的诊断标准。所有士兵的DEN IgM抗体均为阴性，其中10人具有与DEN抗原反应的黄病毒IgG抗体。这些数据表明，VEE ID病毒是秘鲁士兵中引起疾病​​的原因之一，这是该VEE亚型与秘鲁人类疾病的首次关联。

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