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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Mitochondrial DNA variation among Anopheles albimanus populations.
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Mitochondrial DNA variation among Anopheles albimanus populations.

机译:线虫按蚊群体中线粒体DNA变异。

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摘要

Barriers to gene flow between Pacific and Atlantic coast populations of Anopheles albimanus were reported in an earlier study of variation in the intergenic spacer of the nuclear ribosomal DNA. We examined the distribution of mitochondrial DNA haplotypes among A. albimanus populations to test for gene flow barriers with an independent genetic marker. A region of the NADH dehydrogenase subunit 5 gene was amplified by the polymerase chain reaction (PCR) in 1,105 mosquitoes collected from 16 locations in Guatemala and in single collections from Mexico, Honduras, Nicaragua, Costa Rica, Panama, Colombia, and Venezuela. The PCR products were tested for variation using single strand conformation polymorphism analysis and 45 haplotypes were detected. Haplotype frequencies did not vary between coasts in Guatemala. Populations within approximately 200 km of one another were panmictic. However, at distances > 200 km, FST and geographic distances were correlated suggesting that populations are isolated by distance.
机译:在较早的研究中,核糖体DNA的基因间隔区发生了变化,从而报道了太平洋沿大西洋和大西洋沿岸按蚊之间的基因流动障碍。我们检查了线粒体DNA单倍型在A. albimanus人群中的分布,以测试具有独立遗传标记的基因流障碍。通过聚合酶链反应(PCR)在危地马拉16个地点收集的1,105只蚊子中以及从墨西哥,洪都拉斯,尼加拉瓜,哥斯达黎加,巴拿马,哥伦比亚和委内瑞拉的单个收集物中扩增了NADH脱氢酶亚基5基因的一个区域。使用单链构象多态性分析测试PCR产物的变异,并检测到45个单倍型。危地马拉沿岸的单体型频率没有变化。彼此相距约200公里以内的人群为恐慌性人群。但是,在距离> 200 km时,FST和地理距离是相关的,这表明人口是通过距离隔离的。

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