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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding.
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SALP16, a gene induced in Ixodes scapularis salivary glands during tick feeding.

机译：SALP16，是tick食期间在肩I突唾液腺中诱导的基因。

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摘要

Guinea pigs infested with Ixodes scapularis acquire antibody-mediated resistance to tick bites, a phenomenon known as tick-immunity. An I. scapularis salivary gland cDNA expression library was therefore probed with sera from tick-immune guinea pigs to identify antigens that elicit humoral responses in the host. Sera from sensitized guinea pigs strongly recognized 3 of 4,500 library clones in an initial screening. The open reading frames of all 3 clones encoded a putative 16.4-kD acidic protein, designated Salp16, with an N-terminal signal sequence and signal peptidase cleavage sites specific for secretory proteins. The salp16 mRNA and Salp16 protein were detected in the salivary glands of engorged, but not unfed, nymphal and adult ticks, and Salp16 was also found in the saliva of engorged ticks. Immunization with recombinant Salp16 induced high antibody titers in guinea pigs, but did not elicit tick-immunity. Salp16 is the first feeding inducible gene that has been cloned from L. scapularis. Molecular characterization of I. scapularis salivary antigens that are induced upon tick feeding should help to facilitate our understanding of tick-host interactions.
机译：被肩x突螨感染的豚鼠获得了抗体介导的对tick叮咬的抗性，这种现象被称为tick免疫。因此用来自壁虱免疫豚鼠的血清探查肩唾液腺cDNA表达文库，以鉴定引起宿主体液反应的抗原。在最初的筛选中，致敏的豚鼠血清强烈识别4,500个文库克隆中的3个。所有3个克隆的开放阅读框均编码一个推定的16.4-kD酸性蛋白，称为Salp16，具有N端信号序列和分泌蛋白特异的信号肽酶切割位点。在饱腹但未进食的若虫和成年tick的唾液腺中检测到salp16 mRNA和Salp16蛋白，在饱食的s的唾液中也发现Salp16。重组Salp16免疫在豚鼠中诱导了较高的抗体滴度，但未引起tick免疫。 Salp16是第一个从肩cap乳杆菌克隆的进食诱导型基因。 tick喂养后诱导的肩I唾液抗原的分子表征应有助于促进我们对tick-宿主相互作用的理解。

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