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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Diagnosing infection levels of four human malaria parasite species by a polymerase chain reaction/ligase detection reaction fluorescent microsphere-based assay.
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Diagnosing infection levels of four human malaria parasite species by a polymerase chain reaction/ligase detection reaction fluorescent microsphere-based assay.

机译：通过基于聚合酶链反应/连接酶检测反应的荧光微球分析法诊断四种人类疟疾寄生虫的感染水平。

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摘要

Improving strategies for diagnosing infection by the four human Plasmodium species parasites is important as field-based epidemiologic and clinical studies focused on malaria become more ambitious. Expectations for malaria diagnostic assays include rapid processing with minimal expertise, very high specificity and sensitivity, and quantitative evaluation of parasitemia to be delivered at a very low cost. Toward fulfilling many of these expectations, we have developed a post-polymerase chain reaction (PCR)/ligase detection reaction-fluorescent microsphere assay (LDR-FMA). This assay, which uses Luminex FlexMAP microspheres, provides simultaneous, semi-quantitative detection of infection by all four human malaria parasite species at a sensitivity and specificity equal to other PCR-based assays. In blinded studies using P. falciparum-infected blood from in vitro cultures, we identified infected and uninfected samples with 100% concordance. Additionally, in analyses of P. falciparum in vitro cultures and P. vivax-infected monkeys, comparisons between parasitemia and LDR-FMA signal intensity showed very strong positive correlations (r > 0.95). Application of this multiplex Plasmodium species LDR-FMA diagnostic assay will increase the speed, accuracy, and reliability of diagnosing human Plasmodium species infections in epidemiologic studies of complex malaria-endemic settings.
机译：随着针对疟疾的基于现场的流行病学和临床研究变得更加雄心勃勃，改善诊断四种人类疟原虫物种寄生虫感染的策略非常重要。疟疾诊断测定的期望包括以最少的专业知识进行快速处理，非常高的特异性和灵敏度，以及以非常低的成本进行的寄生虫病定量评估。为了满足这些期望中的许多要求，我们开发了一种后聚合酶链反应（PCR）/连接酶检测反应-荧光微球测定（LDR-FMA）。该检测使用Luminex FlexMAP微球，可同时，半定量检测所有四种人类疟原虫物种的感染，其灵敏度和特异性与其他基于PCR的检测相同。在使用恶性疟原虫感染的体外培养血液进行的盲法研究中，我们确定了100％一致性的感染和未感染样品。此外，在恶性疟原虫体外培养物和间日疟原虫感染的猴子的分析中，寄生虫血症和LDR-FMA信号强度之间的比较显示出非常强的正相关（r> 0.95）。在复杂疟疾流行地区的流行病学研究中，这种多重疟原虫物种LDR-FMA诊断测定的应用将提高诊断人类疟原虫物种感染的速度，准确性和可靠性。

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