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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis.
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Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis.

机译：鉴定一种新的基因，该基因编码长柄巴贝斯球菌的分泌抗原1，并评估其在血清学诊断中的用途。

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摘要

Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens.
机译：假定含有分泌的抗原的来自用吉卜赛氏菌感染的狗的血浆免疫的狗的血清用于筛选cDNA表达文库。从分离的克隆中鉴定出编码BgSA1的新基因。用在大肠杆菌中表达的重组BgSA1免疫的小鼠饲养的血清可以识别分子量为59 kDa的天然寄生虫蛋白。与以前建立的以重组P50为抗原的ELISA相比，以重组BgSA1作为抗原的ELISA在检测现场样品时更为灵敏。此外，用抗BgSA1抗体进行的夹心ELISA可以检测来自实验感染了吉氏短杆菌的狗的连续血浆中循环的BgSA1。这些结果表明，BgSA1可能是开发用于检测特异性抗体和循环抗原的诊断测试的有用靶标。

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