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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study.
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Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: A prospective study.

机译：巢式PCR在临床实践中诊断灌木斑疹伤寒的有用性：一项前瞻性研究。

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摘要

The aims of this study were to determine the diagnostic accuracy and clinical usefulness of using nested polymerase chain reaction (PCR) for the diagnosis of scrub typhus through a prospective comparison of nested PCR and indirect immunofluorescent antibody assay (IFA). We conducted a multi-center prospective study of patients who were suffering with possible scrub typhus infection. Whole blood samples were collected for PCR testing, and sera were obtained for serology evaluation using the indirect IFA and the passive hemagglutination assay (PHA). We prospectively studied 135 patients with possible scrub typhus. One hundred eighteen patients were confirmed as having scrub typhus, 7 patients were undetermined, and 10 patients were confirmed as having other diseases. The results of nested PCR assay showed a sensitivity of 82.2% and a specificity of 100%. Ninety-six of the 118 patients were positive for IgM on their admission day. Of the 22 patients who were negative for IgM antibody at admission, 19 had positive results for nested PCR of the buffy coat. The nested PCR assay of the buffy coat is useful as a rapid and reliable test for confirming the diagnosis of scrub typhus.
机译：这项研究的目的是通过嵌套式PCR和间接免疫荧光抗体测定（IFA）的前瞻性比较，确定使用嵌套式聚合酶链反应（PCR）诊断斑疹伤寒的诊断准确性和临床实用性。我们对患有可能的灌木斑疹伤寒感染的患者进行了多中心前瞻性研究。收集全血样本进行PCR测试，并使用间接IFA和被动血凝试验（PHA）获得血清进行血清学评估。我们前瞻性地研究了135例可能患有灌木斑疹伤寒的患者。确认有118例患有斑疹伤寒，不确定的7例，还有10例患有其他疾病。巢式PCR分析的结果显示灵敏度为82.2％，特异性为100％。 118例患者中有96例在入院当天IgM阳性。在入院时IgM抗体阴性的22例患者中，有19例嵌套白膜层PCR阳性。血沉棕黄层的巢式PCR测定法可用作快速可靠的测试，用于确认灌木斑疹伤寒的诊断。

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