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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods.
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Establishment of a mass screening method of sand fly vectors for Leishmania infection by molecular biological methods.

机译：通过分子生物学方法建立沙蝇媒介利什曼原虫感染的大规模筛选方法。

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摘要

Surveillance of the prevalence of Leishmania and its vector, sand fly species, in endemic and surrounding areas is important for prediction of the risk and expansion of leishmaniasis. In this study, a method for the mass screening of sand flies for Leishmania infection was established. This method was applied to 319 field-captured specimens, and 5 positive sand flies were detected. Sand fly species were identified by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) of the18S rRNA gene, and all the positive flies were Lu. hartmanni. Furthermore, cytochrome b (Cyt b) gene sequence analyses identified all the parasites as Endotrypanum species including a probable novel species. Because the method requires minimum effort and can process a large number of samples at once, it will be a powerful tool for studying the epidemiology of leishmaniasis.
机译：对流行和周边地区利什曼原虫及其媒介，沙蝇种的流行情况进行监测，对于预测利什曼病的风险和扩展非常重要。在这项研究中，建立了一种沙蝇大规模筛查利什曼原虫感染的方法。该方法应用于319个野外捕获标本，共检测到5只阳性沙蝇。通过18S rRNA基因的聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）鉴定沙蝇种，所有阳性果蝇均为Lu。哈特曼尼。此外，细胞色素b（Cyt b）基因序列分析确定了所有寄生虫为内胚乳种，包括可能的新种。因为该方法所需的精力最少，并且可以一次处理大量样本，所以它将成为研究利什曼病流行病学的有力工具。

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