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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.
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A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies.

机译：一种属和种特异性的巢式聚合酶链反应疟疾检测测定法，用于流行病学研究。

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摘要

A nested polymerase chain reaction (PCR) assay that uses Plasmodium genus-specific primers for the initial PCR (nest 1) amplification and either genus- or species-specific primers for the nest 2 amplifications was tested on laboratory and field samples. With in vitro cultured Plasmodium falciparum-infected blood samples, it was capable of detecting six parasites/microl of blood using DNA prepared from 25-microl blood spots on filter paper. The assay was evaluated on fingerprick blood samples collected on filter paper from 129 individuals living in a malaria-endemic area in Malaysia. Malaria prevalence by genus-specific nested PCR was 35.6% (46 of 129) compared with 28.7% (37 of 129) by microscopy. The nested PCR detected seven more malaria samples than microscopy in the first round of microscopic examination, malaria in three microscopically negative samples, six double infections identified as single infections by microscopy and one triple infection identified as a double infection by microscopy. The nested PCR assay described is a sensitive technique for collecting accurate malaria epidemiologic data. When coupled with simple blood spot sampling, it is particularly useful for screening communities in remote regions of the world.
机译：在实验室和现场样本上测试了巢式聚合酶链反应（PCR）分析方法，该方法使用疟原虫属特异性引物进行初始PCR（巢1）扩增，而巢属2扩增属属或物种特异性引物进行了测试。使用体外培养的恶性疟原虫感染的血样，它能够使用从滤纸上25微米血斑制备的DNA检测每毫升血液中的六种寄生虫。对从生活在马来西亚疟疾流行地区的129个人的滤纸上采集的指尖血样进行了评估。属特异性巢式PCR的疟疾患病率为35.6％（129例中的46例），而显微镜检查则为28.7％（129例中的37例）。嵌套式PCR在第一轮显微镜检查中检测出的疟疾样本比显微镜检查多出七个疟疾样本，三个显微镜阴性样本中的疟疾样本，六个通过显微镜鉴定为单次感染的双重感染，以及一个通过显微镜鉴定为双重感染的三次感染。所描述的巢式PCR分析是一种用于收集准确的疟疾流行病学数据的灵敏技术。结合简单的血斑采样，它对于筛查世界偏远地区的社区特别有用。

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