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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Adenosine, AMP, and protein phosphatase activity in sandfly saliva.
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Adenosine, AMP, and protein phosphatase activity in sandfly saliva.

机译:sand唾液中的腺苷,AMP和蛋白质磷酸酶活性。

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摘要

As they probe the skin for blood, sand flies inject saliva that prevents hemostasis. Sand fly saliva also promotes leishmaniasis by suppressing immunologic functions of macrophages. Saliva of Phlebotomus papatasi, the vector of Old World cutaneous leishmaniasis, contains adenosine and AMP. We show that Ph. papatasi saliva as well as pure adenosine down-regulate the expression of the inducible nitric oxide (NO) synthase gene in activated macrophages. In addition Ph. papatasi, but not Lutzomyia longipalpis, saliva inhibits the production of NO. Taken together, these data suggest that salivary adenosine is responsible for the down-regulation of NO synthesis. Saliva of both genera Phlebotomus and Lutzomyia contains significant levels of endogenous protein phosphatase-1/2A-like activity that is heat labile, inhibitable by okadaic acid and calyculine a, and does not require divalent cations.
机译:当他们探查皮肤中的血液时,沙蝇会注入唾液来防止止血。沙蝇唾液还通过抑制巨噬细胞的免疫功能促进利什曼病。旧世界皮肤利什曼病的传染媒介,Plebotomus papatasi的唾液中含有腺苷和AMP。我们显示Ph。papatasi唾液以及纯腺苷下调活化巨噬细胞中诱导型一氧化氮(NO)合酶基因的表达。此外,帕帕塔氏菌,而不是长白僵菌,唾液也能抑制NO的产生。综上所述,这些数据表明唾液腺苷负责NO合成的下调。芦苇属和Lutzomyia属的唾液均含有显着水平的内源性蛋白磷酸酶-1 / 2A样活性,该活性不耐热,可被冈田酸和瓜氨酸a抑制,不需要二价阳离子。

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