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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Molecular analysis of Plasmodium falciparum recrudescent malaria infections in children treated with chloroquine in Nigeria.
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Molecular analysis of Plasmodium falciparum recrudescent malaria infections in children treated with chloroquine in Nigeria.

机译：尼日利亚接受氯喹治疗的儿童恶性疟原虫复发性疟疾感染的分子分析。

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Parasite genotyping by a polymerase chain reaction was used to distinguish recrudescent from newly acquired Plasmodium falciparum infections in 50 of 160 Nigerian children taking part in a chloroquine efficacy study in Ibadan, Nigeria. A finger prick blood sample was taken from each child before and after treatment to identify recrudescent parasites. By investigating allelic variation in three polymorphic antigen loci, merozoite surface protein-1 (MSP-1), MSP-2, and glutamate-rich protein (GLURP), we determined parasite diversity in the population and in the infected host. DNA from pretreatment and post-treatment samples from 47 of the 50 patients who failed therapy was successfully amplified by the PCR. The MSP-1, MSP-2, and GLURP genotypes in all samples showed extensive diversity, indicating polyclonal infections. The average number of clones per infection in pre-treatment sample was 2.5 with MSP-1, 4.9 with MSP-2, and 2 with GLURP. The extent of multiplicity decreased significantly (P = 0.016) in posttreatment samples. Multiplicity of infection and initial parasite density were not age dependent. Comparison of the variant alleles in pretreatment and post-treatment samples of each patient indicates that 26 of the 47 children had genuinely recrudescent disease. Conversely, post-treatment samples from five children showed completely new genotypes, indicating either a previously sequestered population of parasites or a newly acquired infection. Overall, this study has shown the diversity and complexity of P. falciparum population in Ibadan, Nigeria. The study has also shown the dynamics of P. falciparum infections in this population before and after chloroquine treatment in an area of high malaria transmission.
机译：在尼日利亚伊巴丹参加氯喹功效研究的160名尼日利亚儿童中，有50名尼日利亚儿童中，通过聚合酶链反应进行的寄生虫基因分型被用于区分新发恶性疟原虫感染与复发。在治疗之前和之后，从每个孩子那里采集手指刺血样品，以鉴定复发性寄生虫。通过研究三个多态性抗原基因座，裂殖子表面蛋白-1（MSP-1），MSP-2和富含谷氨酸的蛋白（GLURP）中的等位基因变异，我们确定了种群和感染宿主中的寄生虫多样性。通过PCR成功扩增了50例治疗失败的患者中47例的治疗前后样本中的DNA。所有样品中的MSP-1，MSP-2和GLURP基因型均表现出广泛的多样性，表明存在多克隆感染。治疗前样品中每次感染的平均克隆数为2.5（使用MSP-1），4.9（使用MSP-2）和2（使用GLURP）。在后处理样品中，多样性程度显着降低（P = 0.016）。感染的多样性和初始寄生虫密度与年龄无关。比较每个患者的治疗前和治疗后样本中的变异等位基因，表明47名儿童中有26名患有真正的复发性疾病。相反，来自五个孩子的治疗后样本显示出全新的基因型，表明以前被隔离的寄生虫种群或新获得的感染。总体而言，这项研究表明尼日利亚伊巴丹恶性疟原虫种群的多样性和复杂性。该研究还显示，在疟疾高发地区，接受氯喹治疗前后该人群恶性疟原虫感染的动态。

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