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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China
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Development of three PCR assays for the differentiation between Echinococcus shiquicus, E. granulosus (G1 genotype), and E. multilocularis DNA in the co-endemic region of Qinghai-Tibet plateau, China

机译：在青藏高原共流行地区区分棘球棘球，虫，颗粒大肠杆菌（G1基因型）和多叶大肠杆菌DNA的三种PCR检测方法的开发

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摘要

To investigate echinococcosis in co-endemic regions, three polymerase chain reaction (PCR) assays based on the amplification of a fragment within the NADH dehydrogenase subunit 1 (ND1) mitochondrial gene were optimized for the detection of Echinococcus shiquicus, Echinococcus granulosus G1, and Echinococcus multilocularis DNA derived from parasite tissue or canid fecal samples. Specificity using parasite tissue-derived DNA was found to be 100% except for E. shiquicus primers that faintly detected E. equinus DNA. Sensitivity of the three assays for DNA detection was between 2 and 10 pg. Ethanol precipitation of negative PCR fecal samples was used to eliminate false negatives and served to increase sensitivity as exemplified by an increase in detection from 0% to 89% of E. shiquicus coproDNA using necropsy-positive fox samples.
机译：为了研究共流行区域的棘球co虫病，优化了基于NADH脱氢酶亚基1（ND1）线粒体基因内片段扩增的三种聚合酶链反应（PCR）分析方法，以检测shichincus球菌，Echinococcus granulosus G1和Echinocococcus来源于寄生虫组织或犬粪样品的多眼DNA。发现使用寄生虫组织来源的DNA的特异性为100％，除了能够隐约检测到马E.equus的E. shiquicus引物。三种检测DNA的检测灵敏度在2到10 pg之间。阴性PCR粪便样品的乙醇沉淀可用于消除假阴性，并有助于提高灵敏度，这可以通过使用尸检阳性狐狸样品将shiquicus coproDNA的检出率从0％提高到89％来证明。

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