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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples.
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Detection of Salmonella typhi by nested polymerase chain reaction in blood, urine, and stool samples.

机译：通过巢式聚合酶链反应在血液，尿液和粪便样品中检测伤寒沙门氏菌。

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摘要

A nested polymerase chain reaction (PCR) specific for Salmonella enterica serovar Typhi was used for the detection of the pathogen in blood, urine, and stool samples from 131 patients with clinical suspicion of typhoid fever. The sensitivity of blood culture, the PCRs with blood, urine, and feces, and the Widal test were 61.8%, 84.5%, 69.3%, 46.9%, and 39.0%, respectively. The sensitivity of the PCRs with blood (P < 0.001) and urine (P = 0.01) were significantly higher, and the sensitivity of the PCR with feces (P > 0.05) was similar to that of blood culture. Combined, the PCRs on urine and feces showed positive results for 16 (70%) of 23 typhoid patients with negative results with blood culture and PCR with blood. These results show that the PCR with blood is a sensitive method for the diagnosis of typhoid fever, and that the PCRs with urine and feces could be useful complementary tests.
机译：巢式聚合酶链反应（PCR）专用于肠道沙门氏菌血清型伤寒沙门氏菌，用于检测131例临床怀疑伤寒患者的血液，尿液和粪便样本中的病原体。血液培养的敏感性，血液，尿液和粪便的PCR以及Widal检验的敏感性分别为61.8％，84.5％，69.3％，46.9％和39.0％。血液（P <0.001）和尿液（P = 0.01）的PCR敏感性显着更高，粪便（P> 0.05）的PCR敏感性与血液培养相似。结合起来，尿液和粪便的PCR结果显示23例伤寒患者中有16例（70％）阳性，而血液培养和PCR阴性。这些结果表明，血液PCR是诊断伤寒的灵敏方法，尿液和粪便的PCR可作为有用的补充检测。

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