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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Identification of cryptosporidium species infecting humans in Tunisia.
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Identification of cryptosporidium species infecting humans in Tunisia.

机译：在突尼斯感染人类的​​隐孢子虫种类的鉴定。

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摘要

Prevalence and species distribution of Cryptosporidium spp. were determined among 633 immunocompetent children less than five years of age and 75 patients hospitalized for immunodeficiency who lived in northern Tunisia. Microscopy was used for initial screening to detect positive samples and a nested polymerase chain reaction and restriction fragment length polymorphism analysis was used to determine the species. Cryptosporidium spp. was identified in 2.7% of cases (19 stool samples), and there was a significant difference between samples collected from immunocompromised patients and those collected from healthy children (10.7% versus 1.7%). Prevalence was also significantly higher in diarrheal specimens than in formed specimens (6.3% versus 1.6%). Cryptosporidium hominis and C. parvum were responsible for most Cryptosporidium spp. infections (78.9%). Cryptosporidium hominis was more prevalent in children from urban areas than in those from rural areas, and C. parvum was found with similar prevalence rates in the two populations. Cryptosporidium meleagridis was identified in four children on farms.
机译：隐孢子虫的流行和种类分布。在突尼斯北部居住的633名5岁以下的有免疫能力的儿童和75名因免疫缺陷而住院的患者中进行了检测。使用显微镜进行初始筛选以检测阳性样品，并使用巢式聚合酶链反应和限制性片段长度多态性分析确定物种。隐孢子虫在2.7％的病例（19个粪便样本）中发现了这种病毒，并且从免疫受损患者收集的样本与从健康儿童收集的样本之间存在显着差异（10.7％对1.7％）。腹泻标本的患病率也显着高于成形标本（6.3％比1.6％）。人隐孢子虫和小隐孢子虫是大多数隐孢子虫属的原因。感染（78.9％）。隐孢子虫在城市地区的儿童中比在农村地区的儿童中更普遍，在这两个人群中发现小隐孢子虫的患病率相似。在农场的四个孩子中发现了隐孢子虫。

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