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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Is real-time polymerase chain reaction (PCR) more useful than a conventional PCR for the clinical management of leishmaniasis?
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Is real-time polymerase chain reaction (PCR) more useful than a conventional PCR for the clinical management of leishmaniasis?

机译：对于利什曼病的临床管理，实时聚合酶链反应（PCR）是否比常规PCR有用？

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摘要

It is currently unknown if the use of a real-time polymerase chain reaction (PCR) adds value to the diagnosis and follow-up prognosis of patients affected by leishmaniasis. We performed a study using a real-time PCR directed against the alpha-polymerase gene and a semiquantitative PCR that target the SSU ribosomal RNA (rRNA) gene as control for the diagnosis and quantification of parasites in patients with visceral (VL) and cutaneous (CL) leishmaniasis. Our single copy real-time PCR missed one diagnosis of VL compared with the conventional PCR, whereas both PCR methods were able to detect Leishmania parasites in CL. Under anti-leishmania treatment the kinetics of parasitemia were comparable with the two methods. The real-time PCR directed against alpha-polymerase of Leishmania despite being able to make a more accurate quantification of parasites does not add to the decision-making management compared with a semiquantitative PCR, and it is comparatively expensive.
机译：目前尚不清楚实时聚合酶链反应（PCR）的使用是否会为利什曼病患者的诊断和随访预后增加价值。我们使用针对α聚合酶基因的实时PCR和针对SSU核糖体RNA（rRNA）基因的半定量PCR作为对照，对内脏（VL）和皮肤（ CL）利什曼病。与常规PCR相比，我们的单拷贝实时PCR漏诊了一项VL诊断，而两种PCR方法均能够检测CL中的利什曼原虫。在抗利什曼原虫治疗下，寄生虫病的动力学与两种方法相当。尽管能够对寄生虫进行更精确的定量，但针对利什曼原虫的α-聚合酶的实时PCR与半定量PCR相比并没有增加决策管理的成本，而且相对昂贵。

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