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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Hookworm (Necator americanus) larval enzymes disrupt human vascular endothelium.
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Hookworm (Necator americanus) larval enzymes disrupt human vascular endothelium.

机译：钩虫（Necator americanus）的幼虫酶破坏人的血管内皮。

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摘要

Knowledge of the molecular mechanisms used by Necator americanus larvae to penetrate the human skin and the vasculature would aid the development of effective vaccines against this important pathogen. In this work, the impact of N. americanus exsheathing fluid (EF) and excretory/secretory products (ES) on the endothelial barrier was examined using human umbilical vein endothelial cells (HUVEC). Cellular responses were assessed by investigating molecular changes at cell-cell junctions and by determining levels of secreted IL-6, IL-8, and vascular endothelial growth factor (VEGF) in the culture medium. It would appear that a repertoire of larval proteases caused a dose-related increase in endothelial permeability as characterized by a decrease in monolayer resistance with increased permeation of tracer-albumin. These barrier changes were associated with disruption of junctional vascular endothelial cadherin (VE-cadherin) and F-actin and an increase in endothelial secretion of IL-6 and IL-8. Our data suggest that larval proteases play an important role in negotiating the endothelium.
机译：了解美洲轮虫幼虫穿透人体皮肤和脉管系统所用的分子机制，将有助于开发针对这种重要病原体的有效疫苗。在这项工作中，使用人脐静脉内皮细胞（HUVEC）检查了美洲猪笼草的排出液（EF）和排泄/分泌产物（ES）对内皮屏障的影响。通过研究细胞-细胞连接处的分子变化并确定培养基中分泌的IL-6，IL-8和血管内皮生长因子（VEGF）的水平来评估细胞反应。幼虫蛋白酶库似乎引起内皮渗透性的剂量相关增加，其特征为单层抗性降低，伴有示踪蛋白-白蛋白渗透增加。这些障碍的改变与结缔血管内皮钙黏着蛋白（VE-cadherin）和F-肌动蛋白的破坏以及IL-6和IL-8的内皮分泌增加有关。我们的数据表明，幼虫蛋白酶在协商内皮中起重要作用。

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