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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Magnetic affinity enzyme-linked immunoassay for diagnosis of schistosomiasis japonicum in persons with low-intensity infection
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Magnetic affinity enzyme-linked immunoassay for diagnosis of schistosomiasis japonicum in persons with low-intensity infection

机译:磁亲和酶联免疫法测定低强度感染者的日本血吸虫病

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摘要

Most schistosome-endemic areas in China are characterized by low-intensity infections that are independent of prevalence. To establish an effective diagnostic method, we developed a magnetic affinity enzyme-linked immunoassay based on soluble egg antigens (SEA-MEIA) for diagnosing schistosomiasis in persons with low-intensity infection with Schistosoma japonicum by comparing it with a conventional enzyme-linked immunosorbent assay (ELISA). Our results showed that the SEA-MEIA had a higher sensitivity and greater precision in the diagnosis of low-intensity S. japonicum infections than the ELISA. In addition, when we used Pearson's correlation in associating SEA-MEIA with ELISA, a significant correlation existed between the two assays (r = 0.845, P < 0.001). Our data indicated that SEA-MEIA, with a higher sensitivity and greater ease of performance, would be valuable for diagnosis of schistosomiasis japonicum in persons with low-intensity infections.
机译:中国大多数血吸虫病流行地区的特点是低强度感染,与流行无关。为了建立有效的诊断方法,我们开发了一种基于可溶性蛋抗原的磁亲和力酶联免疫分析法(SEA-MEIA),通过与常规酶联免疫吸附法进行比较来诊断日本血吸虫病低强度感染者的血吸虫病。 (ELISA)。我们的结果表明,与ELISA相比,SEA-MEIA在诊断低强度日本血吸虫时具有更高的灵敏度和更高的精度。此外,当我们使用Pearson相关性将SEA-MEIA与ELISA关联时,两种检测之间存在显着相关性(r = 0.845,P <0.001)。我们的数据表明,SEA-MEIA具有更高的灵敏度和更易操作的性能,对于诊断低强度感染者的日本血吸虫病具有重要的价值。

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