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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Highly sensitive electrochemical label-free aptasensor based on dual electrocatalytic amplification of Pt-AuNPs and HRP
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Highly sensitive electrochemical label-free aptasensor based on dual electrocatalytic amplification of Pt-AuNPs and HRP

机译：基于Pt-AuNPs和HRP双重电催化扩增的高灵敏度，无电化学标记的适体传感器

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摘要

In this work, a label-free electrochemical aptamer-based sensor (aptasensor) was constructed on account of the direct immobilization of redox probes on an electrode surface. For this proposed aptasensor, a gold nanoparticles (AuNPs)-coated electrode was firstly modified with redox probes-nickel hexacyanoferrates nanoparticles (NiHCFNPs) through chemisorption and electrostatic adsorption. Then, platinum-gold alloy nanoparticles (Pt-AuNPs) and horseradish peroxidase (HRP) were respectively assembled onto the modified electrode surface, which formed the multilayer films for amplifying the electrochemical signal of NiHCFNPs and immobilizing thiolated thrombin aptamers (TBAs). In the presence of target thrombin, the TBA on the multilayer could catch the thrombin onto the electrode surface, which resulted in a barrier for electro-transfer, leading to the decrease of the electrochemical signal of NiHCFNPs amplified by the Pt-AuNPs and HRP toward H_2O_2. The proposed method avoided the redox probes labeling process, increased the amount of redox probes, and further amplified the electrochemical signal. Thus, the approach showed a high sensitivity and a wider linearity to thrombin in the range between 0.01 nM and 50 nM with a detection limit of 6.3 pM.
机译：在这项工作中，由于氧化还原探针直接固定在电极表面上，因此构建了无标记的基于电化学适体的传感器（aptasensor）。对于此拟议的适体传感器，首先通过化学吸附和静电吸附，用氧化还原探针-六氰合铁酸镍纳米颗粒（NiHCFNPs）修饰了金纳米颗粒（AuNPs）涂覆的电极。然后，将铂金合金纳米粒子（Pt-AuNPs）和辣根过氧化物酶（HRP）分别组装到修饰的电极表面，形成多层膜以放大NiHCFNPs的电化学信号并固定化硫醇化凝血酶适体（TBAs）。在存在目标凝血酶的情况下，多层膜上的TBA可以将凝血酶捕获到电极表面，从而形成电转移的屏障，从而导致Pt-AuNPs和HRP扩增的NiHCFNPs的电化学信号向H_2O_2。所提出的方法避免了氧化还原探针的标记过程，增加了氧化还原探针的数量，并进一步放大了电化学信号。因此，该方法显示出对凝血酶的高灵敏度和更宽的线性度，介于0.01 nM和50 nM之间，检测极限为6.3 pM。

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