Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase

Mie M.; Bich Thuy N.P.; Kobatake E.

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Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase

机译：利用蛋白A融合片段海肾荧光素酶开发均相免疫测定系统

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A homogeneous immunoassay system was developed using fragmented Renilla luciferase (Rluc). The B domain of protein A was fused to two Rluc fragments. When complexes between an antibody and fragmented Rluc fusion proteins bind to target molecules, the Rluc fragments come into close proximity and the luminescence activity of fragmented Rluc is restored by complementation. As proof-of-principle, this fragmented Rluc system was used to detect E. coli homogeneously using an anti-E. coli antibody. This journal is

机译：使用片段性海肾荧光素酶（Rluc）开发了均质的免疫分析系统。蛋白A的B结构域与两个Rluc片段融合。当抗体和片段化的Rluc融合蛋白之间的复合物结合到目标分子上时，Rluc片段紧密靠近，片段化的Rluc的发光活性通过互补得以恢复。作为原理的证明，此片段化的Rluc系统用于使用抗E均质检测大肠杆菌。大肠杆菌抗体。这本日记是

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《The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry》 |2012年第5期|共5页
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Mie M.; Bich Thuy N.P.; Kobatake E.;
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正文语种 eng
中图分类分析化学;
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1. Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase [J] . Mie M., Bich Thuy N.P., Kobatake E. The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2012,第5期

机译：利用蛋白A融合片段海肾荧光素酶开发均相免疫测定系统
2. Protein-DNA interaction-based detection of small molecules by employing Renilla luciferase fusion protein: Quantitative and generic measurement of tetracyclines with a Renilla luciferase-tagged Tet repressor protein [J] . Pellinen Teijo, Rytkonen Kalle, Ristiniemi Noora, Analytical Biochemistry: An International Journal of Analytical and Preparative Methods . 2006,第2期

机译：通过使用海肾荧光素酶融合蛋白基于蛋白质-DNA相互作用的小分子检测：用海肾荧光素酶标记的Tet阻遏蛋白对四环素进行定量和通用测定
3. Development of Renilla luciferase complementation system for homogeneous assay [J] . Masayasu Mie, Takashi Andou, Ngo Phan Bich Thuy, Luminescence: The journal of biological and chemical luminescence . 2010,第2期

机译：用于均匀测定的海肾荧光素酶互补系统的开发
4. Development Of Multiprotein Immunoassay System Using Micro Contact Printing And Microfluidic Techniques [C] . Hojatollah Yeganeh, Seyed Mohammad Ahadi, Ali Ziaei ISTM/2007;International symposium on test and measurement . 2007

机译：微接触印刷和微流技术开发多蛋白免疫分析系统
5. In vivo optical imaging of tumors expressing carcinoembryonic antigen (CEA) using engineered antibody fragment-luciferase fusion proteins. [D] . Venisnik, Katy Michelle. 2006

机译：使用工程抗体片段-荧光素酶融合蛋白对表达癌胚抗原（CEA）的肿瘤进行体内光学成像。
6. Split Renilla Luciferase Protein Fragment-assisted Complementation (SRL-PFAC) to Characterize Hsp90-Cdc37 Complex and Identify Critical Residues in Protein/Protein Interactions [O] . Yiqun Jiang, Denzil Bernard, Yanke Yu, 2010

机译：拆分海肾荧光素酶蛋白片段辅助互补（SRL-PFAC）以鉴定Hsp90-Cdc37复合体并鉴定蛋白质/蛋白质相互作用中的关键残基
7. Split Renilla Luciferase Protein Fragment-assisted Complementation (SRL-PFAC) to Characterize Hsp90-Cdc37 Complex and Identify Critical Residues in Protein/Protein Interactions* [O] . Jiang, Yiqun, Bernard, Denzil, Yu, Yanke, 2010

机译：拆分海肾荧光素酶蛋白片段辅助补体（SRL-PFAC）以鉴定Hsp90-Cdc37复合体并鉴定蛋白质/蛋白质相互作用中的关键残基*

Development of a homogeneous immunoassay system using protein A fusion fragmented Renilla luciferase

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