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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Fabrication of micro free-flow electrophoresis chip by photocurable monomer binding microfabrication technique for continuous separation of proteins and their numerical simulation
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Fabrication of micro free-flow electrophoresis chip by photocurable monomer binding microfabrication technique for continuous separation of proteins and their numerical simulation

机译：光固化单体结合微细加工技术用于蛋白质连续分离的微自由流电泳芯片的制备及其数值模拟

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摘要

In this study, a simple, fast, and reliable method to fabricate a micro free-flow electrophoresis (μFFE) device on glass is presented. The two-dimensional depth channel in the chip was easily achieved by using a photocurable monomer (NOA 81) that served as the bonding material. In such a geometrical structure (two-dimensional depth channel), the effect of fluid behavior on the separation efficiency of micro free-flow zone electrophoresis (μFFZE) was simulated. The results of numerical simulation indicate that the pressure at the inlets may play an important role in the separation performance. Under the optimum separation conditions, four FITC-labeled amino acids were well separated, indicating the validity of the performance of the chip. Since the chip was fabricated by organic polymer bonding, it was easily recyclable through a simple re-fabrication process. The reproducibility of results from these recycling re-fabrication chips was investigated. The RSD of the resolution between FITC-l-glycine and FITC-l-phenylalanine was 5.3%. Furthermore, three FITC-labeled proteins were successfully separated with the resolution of 2.2 and 5.46, respectively, by using the coating of neutral liposome.
机译：在这项研究中，提出了一种在玻璃上制造微自由流动电泳（μFFE）装置的简单，快速且可靠的方法。通过使用用作键合材料的光固化单体（NOA 81）可以轻松实现芯片中的二维深度通道。在这种几何结构（二维深度通道）中，模拟了流体行为对微自由流动区电泳（μFFZE）分离效率的影响。数值模拟结果表明，入口处的压力可能对分离性能起重要作用。在最佳分离条件下，四个FITC标记的氨基酸被很好地分离，表明该芯片性能的有效性。由于芯片是通过有机聚合物键合制造的，因此可通过简单的重新制造过程轻松回收。研究了这些循环再造芯片的结果可重复性。 FITC-1-甘氨酸和FITC-1-苯丙氨酸之间的拆分的RSD为5.3％。此外，通过使用中性脂质体涂层，成功分离了三种FITC标记的蛋白，分辨率分别为2.2和5.46。

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