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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Ultrasensitive electrochemical immunosensor for CA 15-3 using thionine-nanoporous gold-graphene as a platform and horseradish peroxidase-encapsulated liposomes as signal amplification
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Ultrasensitive electrochemical immunosensor for CA 15-3 using thionine-nanoporous gold-graphene as a platform and horseradish peroxidase-encapsulated liposomes as signal amplification

机译：以亚硫氨酸-纳米孔金-石墨烯为平台，辣根过氧化物酶包裹的脂质体为信号放大剂的CA 15-3超灵敏电化学免疫传感器

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摘要

This paper describes a novel electrochemical immunosensor using a nanoporous gold (NPG)/graphene (GN) hybrid platform combined with horseradish peroxidase (HRP)-encapsulated liposomes as labels for the sensitive detection of cancer antigen 15-3 (CA 15-3). The electrochemical detection was based on the released HRP from HRP-encapsulated liposomes toward the reduction of H _2O _2 with the help of the thionine (TH) electron mediator. In the presence of CA 15-3, HRP@liposomes and TH-NPG-GN formed a sandwich-type immunocomplex, and the immunocomplex increased with the increment of the CA 15-3 concentration in the sample. The more CA 15-3 antigen in the sample there was, the more HRP@liposomes/anti-CA 15-3 in the immunocomplex there were. Thus, the catalytic current increased. Under optimized conditions, the linear range of the immunoassay is 2 × 10 ~(-5) to 40 U mL ~(-1) with a detection limit of 5 × 10 ~(-6) U mL ~(-1) CA 15-3. The CA 15-3 concentrations of the clinical serum specimens assayed by the developed immunoassay showed consistent results in comparison with those obtained by a commercially available electrochemiluminescence assay. This proposed immunoassay system had many desirable merits including sensitivity, accuracy, and minimal instrumentation required. Significantly, the new protocol may be quite promising, with potentially broad applications for clinical immunoassays.
机译：本文介绍了一种新型的电化学免疫传感器，该传感器使用纳米孔金（NPG）/石墨烯（GN）杂种平台结合辣根过氧化物酶（HRP）包裹的脂质体作为标记，用于敏感检测癌症抗原15-3（CA 15-3）。电化学检测是基于由HRP包封的脂质体释放的HRP，借助硫氨酸（TH）电子介体使H _2O _2还原。在CA 15-3存在的情况下，HRP @脂质体和TH-NPG-GN形成了夹心型免疫复合物，并且随着样品中CA 15-3浓度的增加，免疫复合物也随之增加。样品中的CA 15-3抗原越多，免疫复合物中的HRP @脂质体/抗CA 15-3越多。因此，催化电流增加。在最佳条件下，免疫测定的线性范围为2×10〜（-5）至40 U mL〜（-1），检测极限为5×10〜（-6）U mL〜（-1）CA 15 -3。通过开发的免疫测定法测定的临床血清标本中的CA 15-3浓度与通过市售电化学发光测定法获得的结果相比具有一致的结果。该提议的免疫测定系统具有许多理想的优点，包括灵敏度，准确性和所需的最少仪器。重要的是，新方案可能非常有前途，在临床免疫测定中可能具有广泛的应用。

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