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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Towards single-cell LC-MS phosphoproteomics
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Towards single-cell LC-MS phosphoproteomics

机译：迈向单细胞LC-MS磷酸蛋白质组学

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摘要

Protein phosphorylation is a ubiquitous posttranslational modification, which is heavily involved in signal transduction. Misregulation of protein phosphorylation is often associated with a decrease in cell viability and complex diseases such as cancer. The dynamic and low abundant nature of phosphorylated proteins makes studying phosphoproteome a challenging task. In this review, we summarize state of the art proteomic techniques to study and quantify peptide phosphorylation in biological systems and discuss their limitations. Due to its short-lived nature, the phosphorylation event cannot be precisely traced in a heterogonous cell population, which highlights the importance of analyzing phosphorylation events at the single cell level. Mainly, we focus on the methodical and instrumental developments in proteomics and nanotechnology, which will help to build more accurate and robust systems for the feasibility of phosphorylation analysis at the single cell level. We propose that an automated and miniaturized construction of analytical systems holds the key to the future of phosphoproteomics; therefore, we highlight the benchmark studies in this direction. Having advanced and automated microfluidic chip LC systems will allow us to analyze single-cell phosphoproteomics and quantitatively compare it with others. The progress in the microfluidic chip LC systems and feasibility of the single-cell phosphoproteomics will be beneficial for early diagnosis and detection of the treatment response of many crucial diseases.
机译：蛋白质磷酸化是普遍存在的翻译后修饰，与信号转导密切相关。蛋白质磷酸化的失调通常与细胞活力的降低和诸如癌症的复杂疾病有关。磷酸化蛋白质的动态性和低丰度特性使得研究磷酸化蛋白质组成为一项艰巨的任务。在这篇综述中，我们总结了研究和定量生物系统中肽磷酸化的最新蛋白质组技术，并讨论了它们的局限性。由于其寿命短，因此无法在异源细胞群体中精确追踪磷酸化事件，这突出了在单个细胞水平分析磷酸化事件的重要性。主要地，我们专注于蛋白质组学和纳米技术的方法学和仪器学发展，这将有助于建立更准确和健壮的系统，用于在单细胞水平进行磷酸化分析。我们建议分析系统的自动化和小型化构造是磷酸化蛋白质组学未来的关键。因此，我们重点介绍了这一方面的基准研究。拥有先进的自动化微流控芯片液相色谱系统将使我们能够分析单细胞磷酸化蛋白质组学，并将其与他人进行定量比较。微流控芯片液相色谱系统的进展以及单细胞磷酸化蛋白质组学的可行性将有助于早期诊断和发现许多关键疾病的治疗反应。

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