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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >High-voltage contactless conductivity-detection for lab-on-chip devices using external electrodes on the holder
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High-voltage contactless conductivity-detection for lab-on-chip devices using external electrodes on the holder

机译：使用支架上的外部电极的片上实验室设备的高压非接触电导检测

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摘要

The detection of ionic species in a polymeric planar electrophoresis device by contactless conductivity measurement is described. To our knowledge this is the first report of such measurements carried out with external electrodes which are part of the holder rather than the separation chip itself. The approach allows the use of bare devices as used for optical measurements, which greatly simplifies the method. The use of a sine wave of 100 kHz of a high amplitude of 500 V for cell excitation assures high sensitivity which is demonstrated with electropherograms for alkali and heavy metal ions as well as inorganic anions and carboxylates at concentrations between 10 and 50 muM. The determination of underivatized amino acids was also possible by using a buffer in the alkaline region where these species are present in anionic form. Detection limits were found to be in the order of 1-5 muM for the inorganic ions and between about 5 and 50 muM for the organic species. [References: 22]
机译：描述了通过非接触电导率测量在聚合物平面电泳装置中离子种类的检测。据我们所知，这是使用作为支架一部分而不是分离芯片本身的外部电极进行此类测量的第一个报告。该方法允许使用裸露的设备进行光学测量，从而大大简化了该方法。使用100 kHz正弦波，振幅为500 V的高振幅进行细胞激发可确保很高的灵敏度，这可通过电泳图用于碱金属和重金属离子以及浓度在10至50μM之间的无机阴离子和羧酸盐来证明。还可以通过在碱性区域（其中这些物质以阴离子形式存在）中使用缓冲液来测定未衍生氨基酸。发现检测限对于无机离子为1-5μM，对于有机物质为约5-50μM。 [参考：22]

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