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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Flow injection analysis of mercury(II) based on enzyme inhibition and thermometric detection
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Flow injection analysis of mercury(II) based on enzyme inhibition and thermometric detection

机译：基于酶抑制和测温检测的汞（II）流动注射分析

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摘要

An enzymatic procedure for the determination of mercury(II) is described, based on inhibition of invertase using glucose oxidase and catalase co-immobilised on controlled-pore glass (CPG) coupled to a thermometric continuous-flow sensor system to follow the invertase activity. A small amount of invertase (0.66 u ml~(-1)) was incubated for a short time in sucrose solution and 20 #mu#l of the miture were injected into the enzyme thermistor system to give a temperature change corresponding to 100% enzyme activity. Addition of a mercury(II) sample to the mixture caused a decrease in the invertase activity, that allowed the determination of mercury(II) concentrations in the 5-80 ppb range with RSD <=0.74%. The analysis time was 2-6 min including incubation. The main advantages of this thermometric biosensor assay are as follows: simplicity, with no need for regeneration due to the use of a cheap, soluble sensing enzyme; robustness, with excellent reproductibility and repeatability; and long operational and storage stability of the enzymes involved in the detection system.
机译：描述了一种酶法测定汞（II）的方法，该方法基于使用葡萄糖氧化酶和过氧化氢酶共固定在可控孔玻璃（CPG）上的蔗糖酶抑制作用，该CPG与温度连续流量传感器系统耦合，以追踪蔗糖酶的活性。在蔗糖溶液中将少量的转化酶（0.66 u ml〜（-1））短时间孵育，并将20＃mu＃l的混合物注入酶热敏电阻系统中，以产生对应于100％酶的温度变化活动。向混合物中添加汞（II）样品会导致转化酶活性降低，从而可以测定5-80 ppb范围内的汞（II）浓度，RSD <= 0.74％。包括孵育在内的分析时间为2-6分钟。这种温度生物传感器测定法的主要优点如下：简单，由于使用了廉价的可溶性传感酶而无需再生;坚固耐用，具有出色的可重复性和可重复性;检测系统所涉及的酶具有长期的操作和存储稳定性。

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