...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Rapid flow injection spectrophotometric determination of monofluorophosphates in toothpastes after on-line hydrolysis by alkaline phosphatase immobilized on a cellulose nitrate membrane
【24h】

Rapid flow injection spectrophotometric determination of monofluorophosphates in toothpastes after on-line hydrolysis by alkaline phosphatase immobilized on a cellulose nitrate membrane

机译：硝酸纤维素膜上固定的碱性磷酸酶在线水解后快速流动注射分光光度法测定牙膏中的一氟磷酸盐

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new, rapid flow injection (FI) method is reported for the spectrophotometric determination of monofluorophosphate (MEP) ions in toothpastes. MFP ions are hydrolyzed on-line by alkaline phosphatase (APase) immobilized on a cellulose nitrate membrane, prior to injection in the FI system. The yielded orthophosphate ions are determined spectrophotometrically (#lambda#_(max) = 690 nm) using the molybdenum blue approach. The chemical and FI variables that affected the enzymatic reaction were studied and optimized. A study of interferences was also carried out. The proposed method is very precise (s_r = 0.7% at 1.0 X 10~(-4) mol l~(-1) MFP, n = 12), fast (sampling rate of 72 h~(-1)) and allows the determination of MFP ions in the range of 4.0 X 10~(-5) to 6.0 X 10~(-4) mol l~(-1) with a satisfactory 3#sigma# detection limit of 4.0 X 10~(-6) mol l~(-1). The application of the proposed FI method to toothpaste samples yielded accurate results (e_r < 2.0%) compared with a potentiometric reference procedure.
机译：报道了一种新的快速流动注射（FI）方法，用于分光光度法测定牙膏中的一氟磷酸根（MEP）离子。在注入FI系统之前，MFP离子通过固定在硝酸纤维素膜上的碱性磷酸酶（APase）在线水解。使用钼蓝方法通过分光光度法（λλ_（max）= 690 nm）确定产生的正磷酸盐离子。研究和优化了影响酶促反应的化学和FI变量。还对干扰进行了研究。所提出的方法非常精确（在1.0 X 10〜（-4）mol l〜（-1）MFP下，s_r = 0.7％，n = 12），快速（72 h〜（-1）的采样率）并允许在4.0 X 10〜（-5）至6.0 X 10〜（-4）mol l〜（-1）范围内测定MFP离子，具有令人满意的3＃sigma＃检测限4.0 X 10〜（-6）摩尔l〜（-1）。与电位参考程序相比，所提出的FI方法在牙膏样品上的应用产生了准确的结果（e_r <2.0％）。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号