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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >An electrochemical adaptation of ellman's test
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An electrochemical adaptation of ellman's test

机译：埃尔曼试验的电化学适应

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摘要

The electrochemically initiated reactionof thiols with N,N-diethyl-p-phenylenediamine has been coupled with an existing colorimetric sensing reaction developed by Ellman as a means of providing an electrochemical adaptation of the latter whereby the total thiol species present in a sample can be determined.The detection methodology has been proven to be robust with a linear range for cysteine from 2-120 #mu#M,a limit of detection of 1.17 #mu#M and has shown selectivity against a wide range of potential interferences.The efficiency of the methodology has been examined in the determination and recovery of thiol species in thre growth tissue media,which contain a number of common biological interferences.
机译：硫醇与N，N-二乙基-对苯二胺的电化学引发反应已与Ellman开发的现有比色传感反应耦合，作为提供后者电化学适应性的手段，从而可以确定样品中存在的总硫醇种类事实证明，该检测方法具有很强的鲁棒性，线性范围为2-120＃mu＃M，检测限为1.17＃mu＃M，对多种潜在干扰具有选择性。在确定和回收生长组织培养基中的硫醇种类时，已经对该方法进行了检验，其中含有许多常见的生物干扰物。

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