In-situ monitoring of H2O2 degradation by live cells using voltammetric detection in a lab-on-valve system

Ilkka Lahdesmaki; Young K. Park; Andrea D. Carroll

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In-situ monitoring of H2O2 degradation by live cells using voltammetric detection in a lab-on-valve system

机译：在阀门实验室系统中使用伏安法检测活细胞对H2O2降解的原位监测

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This paper describes a method for monitoring the degradation of hydrogen peroxide by cells immobilized on a beaded support. The detection is based on the voltammetric reduction of hydrogen peroxide on a mercury film working electrode, whilst combining the concept of sequential injection (SI) with the lab-on-valve (LOV) manifold allows the measurements to be carried out in real time and automatically, in well-defined conditions. The method is shown to be capable of simultaneously monitoring hydrogen peroxide in the 10-1000 mu M range and oxygen in the 160-616 mu M range. A correction algorithm has been used to ensure reliable H2O2 results in the presence of varying oxygen levels. The method has been successfully applied to monitoring the degradation of H2O2 by wild-type cells and by catalase-overexpressing mouse embryonic fibroblasts. Since the technique allows the monitoring of the initial response rate, it provides data not accessible by current methods that are end-point-based measurements.

机译：本文描述了一种监测固定在珠状载体上的细胞对过氧化氢降解的方法。该检测基于汞膜工作电极上过氧化氢的伏安还原，同时结合了顺序注入（SI）和阀上实验室（LOV）歧管的概念，可以实时进行测量，并且在定义明确的条件下自动执行。结果表明，该方法能够同时监测10-1000μM范围内的过氧化氢和160-616μM范围内的氧气。已使用一种校正算法来确保在氧气含量变化的情况下可靠的H2O2结果。该方法已成功地用于监测野生型细胞和过氧化氢酶过表达的小鼠胚胎成纤维细胞对H2O2的降解。由于该技术允许监视初始响应率，因此它提供了当前方法（基于端点的测量）无法访问的数据。

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《The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry》 |2007年第8期|共7页
作者
Ilkka Lahdesmaki; Young K. Park; Andrea D. Carroll;
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1. In-situ monitoring of H2O2 degradation by live cells using voltammetric detection in a lab-on-valve system [J] . Ilkka Lahdesmaki, Young K. Park, Andrea D. Carroll The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2007,第8期

机译：在阀门实验室系统中使用伏安法检测活细胞对H2O2降解的原位监测
2. Determination of cadmium in a sequential injection lab-on-valve system with voltammetric detection using a morin modified electrode [J] . Yang Wang, Zaiqing Liu, Jie Tang Analytical methods . 2011,第3期

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3. Determination of cadmium in a sequential injection lab-on-valve system with voltammetric detection using a morin modified electrode [J] . Zaiqing Liu, Jie Tang, Guojun Yao, Analytical methods . 2011,第3期

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4. Bio-Sorption and Speciation of Arsenic Using Renewable Cell-Column in a Lab-on-Valve System with Detection by ETAAS [C] . Min Deng, rnLifan Fei Proceedings of the First Shenyang International Colloquium on Microfluidies . 2007

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机译：使用原位荧光光谱研究聚合物电解质燃料电池中的氧化降解和降解缓解
6. Two-parameter monitoring in a lab-on-valve manifold applied to intracellular H2O2 measurements [O] . Ilkka Lähdesmäki, Petr Chocholouš, Andrea D. Carroll, -1

机译：双参数在上实验室阀组监测施加到细胞内H 2 O 2的测量
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In-situ monitoring of H2O2 degradation by live cells using voltammetric detection in a lab-on-valve system

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