...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection
【24h】

Microscale determination of purines in tissue samples by capillary liquid chromatography with electrochemical detection

机译：毛细管液相色谱-电化学检测法测定组织样品中的嘌呤

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A microscale method for purines involved in intracellular signaling and energy metabolism, including ADP, ATP, cyclic-AMP, NADH and GTP, was developed. The analytes were separated on a fused-silica capillary liquid chromatography column (50 mum inner diameter by 25 cm long) packed with 7 mum reversed-phase particles and detected with a carbon fiber cylinder microelectrode at +1.50 V versus Ag/AgCl reference electrode. With an acetonitrile gradient, the separation was carried out within 15 min. With a 100 nl injection volume, the detection limits varied from 0.9 to 8 fmol depending upon the analyte. The low detection limits make the method suitable for analysis of small tissue samples. As a demonstration of the method, islets of Langerhans were analyzed for their adenosine-related messenger content. [References: 41]
机译：开发了一种嘌呤的微尺度方法，涉及细胞内信号传导和能量代谢，包括ADP，ATP，环状AMP，NADH和GTP。在装有7um反相颗粒的熔融石英毛细管液相色谱柱（内径50 mum，长25 cm）上分离分析物，并用碳纤维圆柱微电极在+1.50 V相对于Ag / AgCl参比电极的条件下进行检测。用乙腈梯度，在15分钟内进行分离。进样量为100 nl时，检测限取决于分析物，范围从0.9到8 fmol。低检测限使该方法适用于分析小组织样本。作为方法的证明，对朗格汉斯岛的腺苷相关信使含量进行了分析。 [参考：41]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号