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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Gene sensors based on peptide nucleic acid (PNA) probes:Relationship between sensor sensitivity and probe/target duplex stability
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Gene sensors based on peptide nucleic acid (PNA) probes:Relationship between sensor sensitivity and probe/target duplex stability

机译：基于肽核酸（PNA）探针的基因传感器：传感器灵敏度与探针/靶标双链体稳定性之间的关系

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摘要

Gene sensors based on peptide nucleic acid (PNA) probes were prepared and the relationship between sensor sensitivity and the duplex stability of the probe PNAs and target complementary DNAs was studied using five synthesized PNAs (10-,15-,17-,20-,and 22-mers).It was found that the association constants for the probe PNA/target DNA pairs depend not only on the length but also on the base pair sequence,and that the trend in the sensor responses was the same as that in the association constants for the corresponding pairs.In addition,by using two kinds of probe PNAs with different lengths,it was demonstrated that fabrication of sensors based on probe PNAs with comparable association constants yielded similar response curves and sensor sensitivities.
机译：制备了基于肽核酸（PNA）探针的基因传感器，并使用五个合成的PNA（10-，15-，17-，20-，和22-聚体）。发现探针PNA /靶DNA对的缔合常数不仅取决于长度，还取决于碱基对序列，并且传感器响应的趋势与此外，通过使用两种不同长度的探针PNA，证明了基于具有可比缔合常数的探针PNA的传感器产生的响应曲线和传感器灵敏度相似。

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