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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots
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Liquid extraction surface analysis field asymmetric waveform ion mobility spectrometry mass spectrometry for the analysis of dried blood spots

机译:液体萃取表面分析场非对称波形离子迁移谱质谱分析干血斑

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摘要

Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the workflow enables gas-phase separation of lipid and protein molecular classes, enabling analysis of both haemoglobin and a range of lipids (phosphatidylcholine or phosphatidylethanolamine, and sphingomyelin species) from a single extraction sample. The work has implications for multiplexed clinical assays of multiple analytes.
机译:液体萃取表面分析(LESA)是一种表面采样技术,可直接从生物底物中进行电喷雾质谱分析。在这里,我们介绍了LESA质谱与高场非对称波形离子迁移谱(FAIMS)结合用于分析滤纸上的干血斑。将FAIMS集成到工作流程中,可以对脂质和蛋白质分子类别进行气相分离,从而可以从单个提取样品中分析血红蛋白和多种脂质(磷脂酰胆碱或磷脂酰乙醇胺和鞘磷脂种类)。这项工作对多种分析物的多重临床分析具有重要意义。

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