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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues
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Use of solid phase extraction for the isolation and clean-up of a derivatised furazolidone metabolite from animal tissues

机译：固相萃取从动物组织中分离和纯化衍生的呋喃唑酮代谢物的用途

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摘要

A method is presented for the determination of proteinbound residues of furazolidone in animal tissue.The use of furazolidone in food-producing animals has been banned in the EU.Illegal use of furazolidone can be monitored most effectively by testing for bound residues containing the 3-amino-2-oxyzolidone (AOZ) moiety which,unlike the parent drug,is stable and can be detected for prolonged periods after cessation of treatment.This paper reports the development of an extraction and clean-up procedure for AOZ from liver using solid phase extraction.The method replaces solvent extraction and provides extensive sample clean-up with removal of approximately 99% of the derivatising agent,2-nitrobenzaldehyde,which may interfere with the determiantion.It also offers the advantage of being suitable for automation,thereby increasing throughput of samples.The extraction procedure may be used for HPLC and ELISA screening echniques.The method has been validated in fortified and incurred pig liver samples,yielding mean recovery of AOZ in excess of 60%.
机译：提出了一种测定动物组织中呋喃唑酮蛋白质结合残基的方法。欧盟已禁止在食用动物中使用呋喃唑酮，通过检测含有3-的结合残基可以最有效地监测呋喃唑酮的非法使用与母体药物不同，氨基-2-氧基唑烷酮（AOZ）成分稳定，在停止治疗后可长期检测。本文报道了使用固相从肝脏中提取和净化AOZ的方法的发展该方法代替了溶剂萃取，并提供了广泛的样品净化功能，去除了约99％的衍生试剂2-硝基苯甲醛，这可能会干扰测定。它还具有适用于自动化的优点，从而提高了通量提取方法可用于HPLC和ELISA筛选技术。该方法已在强化和致病猪肝中进行了验证样品，平均回收率超过60％。

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