...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >An electrochemical DNA hybridization detection assay based on a silver nanoparticle label
【24h】

An electrochemical DNA hybridization detection assay based on a silver nanoparticle label

机译：基于银纳米粒子标记的电化学DNA杂交检测测定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A novel,sensitive electroxchemical DNA hybridization detection assay,using silver nanoparticles as the oligonucleotide labeling tag,is described.The assayrelies on the hybridization of the target DNA with the silver nanoparticle-oligonucleotide DNA probe,follwed by the release of the silver metal atoms anchored on the hybrids by oxidative metal dissolution and the indirect determination of the solubilized Ag~I ions by anodic stripping voltammetry (ASV) at a carbon fiber ultramicroelectrode.The influence of the relevant experimental variables,including the surface coverage of the target oligonucleotide,the duration of the silver dissolution steps and the parameters of the electrochemcial stripping measurement of the silver(I) ions,is examined and optimized.The combination of the remarkable sensitivity of the stripping metal analysis at the microelectrode with the large number of silver(I) ions released from each DNA hybrid allows detection at levels as low as 0.5 pmol L~(-1) of the target oligonucleotides.
机译：描述了一种新颖的，灵敏的电化学DNA杂交检测方法，该方法使用银纳米颗粒作为寡核苷酸标记标签。该检测方法依赖于靶DNA与银纳米颗粒-寡核苷酸DNA探针的杂交，随后释放出锚定的银金属原子碳纤维超微电极上金属氧化物的溶解和阳极溶出伏安法（ASV）间接测定可溶性Ag〜I离子对杂合物的影响考察并优化了银的溶解步骤和银（I）离子的电化学溶出测量参数。结合了在微电极上的溶出金属分析的显着灵敏度和大量的银（I）离子从每个DNA杂种中释放出来的糖可以检测到低至0.5 pmol L〜（-1）的targ等寡核苷酸。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号