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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Rapid,quantitative and sensitive immunochromatographic assay based on stripping voltammetric detection of a metal ion label
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Rapid,quantitative and sensitive immunochromatographic assay based on stripping voltammetric detection of a metal ion label

机译：基于溶出伏安法检测金属离子标记物的快速，定量和灵敏的免疫色谱分析

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摘要

A novel,sensitive immunochromatographic electrochemical biosensor (IEB) which combines an immunochromatographic strip technique with an electrochemical detection technique has been demonstrated.The IEB takes advantages of the speed and low-cost of the conventional immunochromatographic test kits and high-sensitivity of stripping voltammetry.Bismuth ions (Bi~(3+)) have been coupled with the antibody through the bifunctional chelating agent diethylenetriamine pentaacetic acid (DTPA).After immunoreactions,Bi~(3+) was released and quantified by anodic stripping voltammetry at a built-in single-use screen-printed electrode.As an example for the applications of such novel device,the detection of human chorionic gonadotronphin (HCG) in a specimen was performed.This biosensor provides a more user-friendly,rapid,clinically accurate,less expensive immunoassay for such analysis in specimens than currently available test kits.
机译：结合免疫层析试纸技术和电化学检测技术的新型灵敏免疫层析电化学生物传感器（IEB）已被证明。IEB充分利用了常规免疫层析测试试剂盒的速度和低成本以及溶出伏安法的高灵敏度。铋离子（Bi〜（3+））通过双功能螯合剂二亚乙基三胺五乙酸（DTPA）与抗体偶联。免疫反应后，Bi〜（3+）释放并通过阳极溶出伏安法在内置电极上定量。一次性使用的丝网印刷电极。以这种新型设备的应用为例，对标本中的人绒毛膜促性腺激素（HCG）进行了检测。该生物传感器提供了一种更加用户友好，快速，临床准确，便宜的传感器样本中进行此类分析的免疫检测方法要比目前可用的检测试剂盒好。

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