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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Label- and marker-free gene detection based on hybridization-induced conformational flexibility changes in a ferrocene-PNA conjugate probe
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Label- and marker-free gene detection based on hybridization-induced conformational flexibility changes in a ferrocene-PNA conjugate probe

机译：基于二茂铁-PNA共轭探针杂交诱导的构象柔韧性变化的无标记和无标记基因检测

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摘要

We report a strategy for label-free and marker-free gene detection transducing the hybridization event to an electrochemical signal based on the hybridization-induced conformational flexibility change in probe structure. The probe structure was designed to possess a ferrocene moiety as a reporter part and a cysteine moiety as an anchor part at each end of a peptide nucleic acid (PNA) as a recognition part. Electrochemical examination of probe-modified gold electrodes revealed that the ferrocene moiety was placed at the flexible end of the linear probe chain. Upon hybridization with a complementary target DNA, the resultant rigid duplex restricted the ferrocene motion to the electrode surface, causing a decrease in the observed current. The target DNA was detected with the detection limit of 1.44 x 10~(-11) M. Thus the probe functioned as a 'self-reporting probe' and detection of the target DNA was demonstrated without the need for external indicators. Moreover, the sensor electrode was able repeatedly to detect the target DNA by the process of regeneration and could discriminate a mismatched DNA.
机译：我们报告了一种策略，将基于杂交诱导的构象柔性变化的探针结构的杂交事件转换为电化学信号，从而实现无标记和无标记基因检测。探针结构被设计为在肽核酸（PNA）的每个末端具有二茂铁部分作为报告子部分和半胱氨酸部分作为锚定部分作为识别部分。探针修饰金电极的电化学检查表明，二茂铁部分位于线性探针链的柔性末端。与互补靶DNA杂交后，所得的刚性双链体将二茂铁的运动限制在电极表面，导致观察到的电流减少。检测目标DNA的检出限为1.44 x 10〜（-11）M。因此，该探针可作为“自报告探针”使用，无需外部指示剂即可检测目标DNA。而且，传感器电极能够通过再生过程反复检测目标DNA，并且可以辨别错配的DNA。

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