...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Americas Journal of Plant Science and Biotechnology >Evaluation of in Vitro Ground Leaf and Grain Assays to Screen Barley for Resistance to Fusarium Head Blight
【24h】

Evaluation of in Vitro Ground Leaf and Grain Assays to Screen Barley for Resistance to Fusarium Head Blight

机译：评价体外筛选大麦对镰刀霉病的抗性

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ground leaf from the greenhouse grown plants and grain harvested from field were used in in vitro assays to evaluate barley (Hordeum vulgare L.) germplasm for resistance or tolerance to Fusarium head blight (FHB) incited by Fusarium graminearum Schwabe and F. culmorum (W.G Smith) Sacc. On media incorporating ground leaf tissue of eight barley genotypes, F. graminearum displayed significantly larger colonies on medium made from susceptible 'AC Lacombe' and 'Stetson' barley compared to resistant 'Chevron' and 192130. Macroconidial production was significantly reduced on 'Chevron'-based ground leaf tissue medium. In another test including ten barley genotypes, fungal colonies were larger on medium that contained ground grain from susceptible 'AC Lacombe' and 'Stander' barley compared to ground grain from resistant Penco/Chevron, 'Seebe' and 192130; ground grain from other barley cultivars or germplasm supported intermediate fungal growth. Similar results using ground grain from susceptible and resistant lines were obtained with 15 additional breeding lines. The results were repeatable as evidenced by a significant correlation (r = 0.87, P < 0.05) between tests. Ground grain medium prepared from resistant barley germplasm resulted in reduced sporulation by F graminearum compared to that measured using susceptible cultivars. Both the in vitro leaf tissue and ground grain assays resulted in a similar ranking for fungal colony diameter. Fungal growth using the ground leaf or ground grain assay was correlated with FHB field reactions for a small number of barley lines (r = 0.69, P < 0.05; r = 0.56, P < 0.05), but was not correlated with field FHB reactions that involved a larger number of genotypes. The significance of the two in vitro assays is discussed in relation to screening for FHB resistance.
机译：大棚植物的地面叶片和田间收获的谷物用于体外测定，以评估大麦（Hordeum vulgare L.）种质对禾谷镰刀菌（Fusarium graminearum Schwabe）和F. culmorum（WG）引起的枯萎病（Fusarium head blight）（FHB）的抗性或耐受性史密斯（Smith）在整合了八种大麦基因型地面叶片组织的培养基上，禾谷镰刀菌在易感的“ AC Lacombe”和“ Stetson”大麦制成的培养基上显示出明显的集落，而抗药性“ Chevron”和192130则大。的地面叶组织培养基。在另一个包含十种大麦基因型的试验中，与来自抗药性Penco / Chevron，“ Seebe”和192130的谷粒相比，含有易感“ AC Lacombe”和“ Stander”大麦的谷粒的培养基上的真菌菌落更大。其他大麦品种或种质的地面谷物支持中间真菌的生长。使用15个额外的育种系，从易感和抗病系获得的地面谷物获得了相似的结果。测试之间的显着相关性（r = 0.87，P <0.05）证明了结果是可重复的。与使用易感品种测得的相比，由抗性大麦种质制得的地面谷物培养基可降低禾谷镰孢的孢子形成。体外叶片组织和地面颗粒分析均得出真菌菌落直径相似的排名。对于少量大麦系，使用地面叶或地面谷物测定法进行的真菌生长与FHB田间反应相关（r = 0.69，P <0.05; r = 0.56，P <0.05），但与田间FHB反应无关涉及大量的基因型。讨论了两种体外测定法与FHB耐药性筛查的意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号