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首页> 外文期刊>The Americas Journal of Plant Science and Biotechnology >Tetranucleotide and Dinucleotide Microsatellite Markers for Red Spruce (Picea rubens)
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Tetranucleotide and Dinucleotide Microsatellite Markers for Red Spruce (Picea rubens)

机译：红云杉的四核苷酸和二核苷酸微卫星标记（Picea rubens）

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摘要

Three library enrichment protocols were used to develop tetra-and dinucleotide microsatellite markers for red spruce (Picea rubens). Two enrichment methods based on a single round of magnetic beads-based subtractive hybridization showed limited success. A third method with two successive rounds of subtractive hybridization had a high enrichment success but was found to increase the rate of redundancy and chimerical sequences among the clone sequences. Several single locus tetra- and dinucleotide markers were discovered and can be useful in mapping studies. All newly developed markers except one were characterized by a high frequency of null alleles. One new and one previously described microsatellite marker were identified as informative, reliable and characterized by a low null allele frequency, and were suitable for paternity/parentage and population genetic analyses in red spruce.
机译：三种文库富集规程用于开发红云杉（Picea rubens）的四核苷酸和二核苷酸微卫星标记。基于单轮基于磁珠的减性杂交的两种富集方法显示出有限的成功。具有连续两轮减性杂交的第三种方法具有较高的富集成功率，但是发现该方法可提高克隆序列中的冗余序列和嵌合序列。发现了几个单基因座的四核苷酸和二核苷酸标记，可用于作图研究。除一个标记外，所有新开发的标记均以高频率的无效等位基因为特征。一种新的和一种先前描述的微卫星标记被鉴定为信息量大，可靠且具有低无效等位基因频率的特征，并且适用于红色云杉的亲子/亲本和群体遗传分析。

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