...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Ca2+ regulation of a large conductance K+ channel in cultured rat cerebellar Purkinje neurons.
【24h】

Ca2+ regulation of a large conductance K+ channel in cultured rat cerebellar Purkinje neurons.

机译：培养的大鼠小脑浦肯野神经元中大电导K +通道的Ca2 +调节。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The calcium sensitivity of a large conductance voltage-sensitive potassium channel found in cultured rat cerebellar Purkinje neurons was studied in membrane patches from the somatic region of the cultured Purkinje neurons using single-channel recording techniques. The potassium channel had a conductance of approximately 94 picosiemens (pS) under physiologic ionic conditions and was active at depolarized membrane potentials. Activity due to this channel type was not observed when the saline at the internal surface of the membrane was calcium free. Low intracellular calcium concentration (10 nM) triggered channel activity at depolarized membrane potentials. Channel activity increased further with increasing intracellular calcium concentrations and was evident at more negative membrane potentials. The high sensitivity of this potassium channel type to intracellular calcium and its abundance in the Purkinje neuron membrane may reflect a prominent role in the control of action potential duration and interspike interval when the neurons are firing in a rapid, repetitive mode.
机译：使用单通道记录技术，在培养的小脑浦肯野神经元的体细胞膜片中研究了在培养的大鼠小脑浦肯野神经元中发现的大电导电压敏感钾通道的钙敏感性。钾离子通道在生理离子条件下的电导率约为94皮考曼（pS），并在去极化膜电位下具有活性。当膜内表面的盐水不含钙时，未观察到由于该通道类型引起的活性。低细胞内钙浓度（10 nM）触发了去极化膜电位下的通道活性。通道活性随着细胞内钙浓度的增加而进一步增加，并且在更大的负膜电位下很明显。这种钾通道类型对细胞内钙的高度敏感性及其在浦肯野神经元膜中的丰富度，可能反映了当神经元以快速，重复的方式发射时，在控制动作电位持续时间和峰间间隔方面的重要作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号