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首页> 外文期刊>The European Journal of Neuroscience >Xenopus muscle-specific kinase: molecular cloning and prominent expression in neural tissues during early embryonic development.
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Xenopus muscle-specific kinase: molecular cloning and prominent expression in neural tissues during early embryonic development.

机译：爪蟾肌肉特异性激酶：早期胚胎发育过程中神经组织中的分子克隆和突出表达。

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摘要

A muscle-specific receptor tyrosine kinase, designated MuSK, mediates agrin-induced aggregation of acetylcholine receptors at the vertebrate neuromuscular junction. cDNAs encoding Xenopus MuSK were isolated from embryonic cDNA libraries. The full-length MuSK cDNA encodes for a polypeptide of 948 amino acids and possesses the features unique to mammalian MuSK, including four Ig-like domains, C6 box, transmembrane region and an intracellular tyrosine kinase domain. Interestingly, Xenopus MuSK also contains a kringle domain similar to that previously reported for Torpedo MuSK. The overall amino acid sequence identity of Xenopus MuSK with mammalian MuSK is approximately 65%. Northern blot analysis demonstrated the presence of three MuSK transcripts (approximately 1 kb, approximately 3 kb and approximately 7 kb) which were differentially expressed during development. The expression of the approximately 7 kb MuSK transcript remained as the predominant species in adult tissues, e.g. skeletal muscle, spleen and lung. Immunocytochemical analysis with a MuSK-specific antibody revealed that Xenopus MuSK was colocalized with AChRs at neuromuscular junctions as well as in spontaneous acetylcholine receptor hot spots of cultured muscle cells. In situ hybridization revealed prominent expression of MuSK transcripts in neural tissues and myotomal muscle during the period of neurulation and synaptogenesis. The MuSK transcript detected at abundant levels in the central nervous system (CNS) was localized to the brain, spinal cord and eye vesicles during early embryonic development. In addition, the MuSK protein in the developing eye was found to be prominently expressed during embryonic stages of 32 and 35. These findings raise an intriguing possibility that, in addition to the known function in the formation of the neuromuscular junctions, MuSK may be involved in neural development.
机译：一种肌肉特异性受体酪氨酸激酶，称为MuSK，在脊椎动物神经肌肉接头处介导凝集素诱导的乙酰胆碱受体聚集。从胚胎cDNA文库中分离出编码非洲爪蟾MuSK的cDNA。全长MuSK cDNA编码948个氨基酸的多肽，并具有哺乳动物MuSK特有的特征，包括四个Ig样结构域，C6框，跨膜区和细胞内酪氨酸激酶结构域。有趣的是，非洲爪蟾MuSK还包含一个类似于先前针对Torpedo MuSK报道的kringle域。非洲爪蟾MuSK与哺乳动物MuSK的总体氨基酸序列同一性约为65％。 Northern印迹分析表明存在三个在发育过程中差异表达的MuSK转录本（约1 kb，约3 kb和约7 kb）。大约7 kb MuSK转录本的表达仍然是成人组织中的主要种类，例如骨骼肌，脾脏和肺脏。用MuSK特异性抗体进行的免疫细胞化学分析显示，非洲爪蟾MuSK与AChRs共同定位在神经肌肉连接处以及培养的肌肉细胞的自发乙酰胆碱受体热点中。原位杂交揭示了在神经元和突触形成期间，MuSK转录物在神经组织和肌肌中显着表达。在早期胚胎发育过程中，在中枢神经系统（CNS）中大量检测到的MuSK转录物位于大脑，脊髓和眼囊。此外，发现发育中的眼睛中的MuSK蛋白在32和35的胚胎阶段显着表达。这些发现引起了一种有趣的可能性，即除了已知的在神经肌肉接头形成中的功能外，MuSK也可能参与其中。在神经发育中。

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