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首页> 外文期刊>The European Journal of Neuroscience >Neurotrophins BDNF and NT-3 promote axonal re-entry into the distal host spinal cord through Schwann cell-seeded mini-channels.
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Neurotrophins BDNF and NT-3 promote axonal re-entry into the distal host spinal cord through Schwann cell-seeded mini-channels.

机译：Neurotrophins BDNF和NT-3通过许旺细胞播种的微通道促进轴突重新进入远端宿主脊髓。

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摘要

To promote axonal regeneration in the injured adult spinal cord, a two-phase repair strategy was employed to (i) bridge a spinal cord hemilesion cavity with a grafted Schwann cell (SC)-seeded mini-channel, and (ii) promote axonal re-entry into the distal cord by infusing two neurotrophins, BDNF and/or NT-3, directly into the distal cord parenchyma. Here we report that infusion of two neurotrophins, delivered alone or in combination, effectively promotes axonal outgrowth from SC-seeded mini-channels into the distal host spinal cord. When an anterogradely transported marker, PHA-L or BDA, was injected into the spinal cord 3 mm rostral to the graft, a large number of axons was observed to regenerate from the SC graft into the distal cord in neurotrophin-treated groups. A subpopulation of these axons was found to grow up to 6 mm within the distal spinal cord. These axons, which were confined mainly within the grey matter, arborized and formed structures which resemble terminal boutons. In channels containing no SCs, the infusion of neurotrophins did not promote axonal ingrowth from the proximal cord stump. In cases which received SC grafts but no neurotrophin infusion, axonal re-entry into the distal cord was limited. Thus, the present study demonstrates that regenerating axons not only cross a lesion site when a permissive cellular bridge is provided but also penetrate into the distal host spinal cord and elongate for a distance of several cord segments after the infusion of two neurotrophins. The latter event is prerequisite for establishment of appropriate connections between regenerating axons and target neurons and thus, functional recovery.
机译：为了促进成年脊髓损伤中的轴突再生，采用了两阶段修复策略，以（i）用植入了施万旺细胞（SC）的微型通道弥合脊髓的横断腔，以及（ii）促进轴突重建。 -通过将两种神经营养蛋白BDNF和/或NT-3直接注入远端脊髓实质进入远端脊髓。在这里，我们报道了两种单独或组合给药的神经营养素的输注可有效促进轴突从SC播种的微通道向远端宿主脊髓的轴突生长。当将顺行转运的标记物PHA-L或BDA注射到与移植物相距3 mm的脊髓中时，在神经营养蛋白治疗组中观察到大量轴突从SC移植物再生到远端脊髓。发现这些轴突的亚群在远端脊髓内长至6 mm。这些轴突主要局限在灰质中，形成并形成类似于末端纽扣的结构。在不含SC的通道中，输注神经营养蛋白不会促进近端残端轴突向内生长。在接受SC移植但未输注神经营养蛋白的情况下，轴突再次进入远端脊髓受到限制。因此，本研究表明，再生轴突不仅在提供允许的细胞桥时穿过病变部位，而且在注入两种神经营养蛋白后可穿透远端宿主脊髓并延伸一段距离。后一事件是在再生轴突和目标神经元之间建立适当连接并因此恢复功能的先决条件。

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