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首页> 外文期刊>The European Journal of Neuroscience >Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat.
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Synaptic contacts of vesicular glutamate transporter 2 fibres on chemically identified neurons of the hypothalamic suprachiasmatic nucleus of the rat.

机译：水泡谷氨酸转运蛋白2纤维在大鼠的下丘脑上视交叉上核的神经元上的突触接触。

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摘要

The hypothalamic suprachiasmatic nucleus (SCN), which plays a pivotal role in the control of circadian rhythms, consists of several neuronal subpopulations characterized by different neuroactive substances. This prominent cell group has a fairly rich glutamatergic innervation, but the cell types that are targeted by this innervation are unknown. Therefore, the purpose of the present study was to examine the relationship between the afferent glutamatergic axon terminals and the vasoactive intestinal polypeptide (VIP)-, arginine-vasopressin (AVP)- and gamma-aminobutyric acid (GABA)-positive neurons of the SCN. Glutamatergic elements were revealed via immunocytochemical double-labelling for vesicular glutamate transporter type 1 (VGluT1) and type 2 (VGluT2), and brain sections were imaged via confocal laser-scanning microscopy and electron microscopy. Numerous VGluT2-immunoreactive axons were observed to be in synaptic contact with VIP- and GABA-positive neurons, and only a few synapses were detected between VGluT2 boutons and AVP neurons. VGluT1 axon terminals exhibiting very moderate distribution in this cell group were observed to be in synaptic contact with chemically unidentified neurons. The findings provide the first morphological data on the termination of presumed glutamatergic fibres on chemically identified neurons of the rat SCN, and indicate that all three prominent cell types of the cell group receive glutamatergic afferents.
机译：下丘脑上视交叉核（SCN）在昼夜节律的控制中起着关键作用，由几个神经元亚群组成，这些亚群的特征在于不同的神经活性物质。这个突出的细胞群具有相当丰富的谷氨酸能神经支配，但是这种神经支配靶向的细胞类型是未知的。因此，本研究的目的是检查传入的谷氨酸能轴突末端与SCN的血管活性肠多肽（VIP），精氨酸血管加压素（AVP）和γ-氨基丁酸（GABA）阳性神经元之间的关系。 。通过免疫细胞化学双重标记揭示了1型水泡谷氨酸转运蛋白（VGluT1）和2型水泡谷氨酸转运蛋白（VGluT2）的谷氨酸能元素，并通过共聚焦激光扫描显微镜和电子显微镜对大脑切片进行了成像。观察到许多VGluT2免疫反应性轴突与VIP和GABA阳性神经元发生突触接触，并且在VGluT2钮扣和AVP神经元之间仅检测到少数突触。 VGluT1轴突终端表现出非常适度的分布，在此细胞组中观察到与化学未鉴定的神经元突触接触。这些发现提供了关于在大鼠SCN的化学鉴定神经元上推测的谷氨酸能纤维终止的第一个形态学数据，并表明该细胞群的所有三种主要细胞类型都接受了谷氨酸能传入。

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