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首页> 外文期刊>The European Journal of Neuroscience >Spontaneous regeneration of intrinsic spinal cord axons in a novel spinal cord slice culture model.
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Spontaneous regeneration of intrinsic spinal cord axons in a novel spinal cord slice culture model.

机译：在新型的脊髓切片培养模型中固有脊髓轴突的自发再生。

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A substantial problem in research concerned with axonal repair is the use of a wide variety of lesion models and the complexity associated with the respective in vivo lesion paradigms. Organotypic slice cultures are a potential in vitro alternative because the cytoarchitectonic tissue organization is well preserved and the slices can be maintained in culture for several weeks. Until now no spinal cord slice culture model for the study of axonal growth has been available. Here we present a spinal cord slice culture model that is well suited for the study of axonal growth. The spinal cord slices were cut not in the transverse but in the sagittal longitudinal plane such that several spinal cord segments were included in the slice culture. In these cultures the typical ventro-dorsal polarity of the spinal cord was maintained and intrinsic spinal cord axons formed a strong fibre tract extending along the longitudinal axis of the slice. The axons became myelinated during the culture period and synaptic contacts were present in these cultures. After mechanical lesions the intrinsic spinal cord axons had a substantial potential for axonal growth and regeneration. The number of regenerating axons crossing the lesion site decreased with increasing maturation of the culture, but even in mature cultures a small number of crossing fibres were present. This slice culture model could provide an important tool for several aspects of spinal cord research in the fields of axonal growth and regeneration, synapse formation, formation of intrinsic spinal cord circuits and myelination.
机译：与轴突修复有关的研究中的一个实质性问题是使用多种病变模型以及与各自的体内病变范例相关的复杂性。器官型切片培养是一种潜在的体外替代方法，因为细胞建筑师组织的组织得到了很好的保存，并且切片可以在培养物中维持数周。迄今为止，还没有用于轴突生长研究的脊髓切片培养模型。在这里，我们提出了一种非常适合于轴突生长研究的脊髓切片培养模型。不在横切面而是在矢状纵切面上切开脊髓切片，以使切片培养物中包括几个脊髓节段。在这些培养物中，维持了脊髓的典型腹背极性，并且固有的脊髓轴突形成了沿着切片的纵轴延伸的强纤维束。在培养期间，轴突变得髓鞘化，并且在这些培养物中存在突触接触。机械损伤后，固有的脊髓轴突具有巨大的轴突生长和再生潜能。随着培养物的成熟度增加，穿过病变部位的再生轴突的数量减少，但是即使在成熟的培养物中，也存在少量的交叉纤维。这种切片培养模型可以为轴突生长和再生，突触形成，固有脊髓回路的形成和髓鞘形成等领域的脊髓研究的多个方面提供重要工具。

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