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首页> 外文期刊>The European Journal of Neuroscience >Lateral habenula projections to dopamine and GABA neurons in the rat ventral tegmental area.
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Lateral habenula projections to dopamine and GABA neurons in the rat ventral tegmental area.

机译：大鼠腹侧被盖区侧ha突向多巴胺和GABA神经元。

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摘要

Ventral tegmental area (VTA) dopamine (DA) neurons and their forebrain projections are critically involved in reward processing and cognitive functions. Descending projections from the lateral habenula (LHb) play a central role in inhibiting DA cell activity in response to the absence of expected rewards. As LHb efferents are reportedly glutamatergic, their ability to inhibit DA cells would theoretically require a disynaptic connection involving VTA GABA neurons and their local collateral inputs to DA cells. We therefore used anterograde tract-tracing from the LHb to investigate the relative selectivity of LHb synapses onto GABA versus DA VTA neurons. LHb axons were visualized using immunoperoxidase, and DA and GABA cells were marked by immunogold-silver labeling for tyrosine hydroxylase (TH) or GABA, respectively. By ultrastructural analysis, 16% of LHb axons were observed to form synaptic contacts in the VTA, and most of these were of an intermediate morphological type that did not exhibit definitive asymmetric or symmetric character. LHb axons synaptically targeted TH- and GABA-labeled dendrites to a comparable extent (45 and 52% observed incidence, respectively). Pre-embedding immunogold labeling for the vesicular glutamate transporter type 2 and post-embedding immunogold staining for GABA confirmed that approximately 85% of LHb terminals were glutamatergic and not GABAergic. These results suggest that the robust inhibition of DA cells evoked by the LHb is unlikely to arise from a selective innervation of VTA GABA neurons. Moreover, the LHb may mediate a direct excitation of DA cells that is over-ridden by indirect inhibition originating from an extrinsic source.
机译：腹侧被盖区（VTA）多巴胺（DA）神经元及其前脑投射关键参与奖励过程和认知功能。由于缺乏预期的奖励，来自外侧哈贝拉（LHb）的下降投影在抑制DA细胞活性中起着核心作用。由于据报道LHb传出是谷氨酸能的，因此它们抑制DA细胞的能力在理论上需要涉及VTA GABA神经元及其对DA细胞的局部附带输入的突触连接。因此，我们使用了来自LHb的顺行道来研究LHb突触对GABA与DA VTA神经元的相对选择性。 LHb轴突使用免疫过氧化物酶可视化，DA和GABA细胞分别通过免疫金银标记酪氨酸羟化酶（TH）或GABA进行标记。通过超微结构分析，观察到16％的LHb轴突在VTA中形成突触接触，并且大多数是中间形态类型，没有表现出确定的不对称或对称特征。 LHb轴突突触地靶向TH和GABA标记的树突（可观察到的发生率分别为45％和52％）。包埋前免疫金标记的2型囊状谷氨酸转运蛋白和包埋后免疫金染色的GABA证实大约85％的LHb末端是谷氨酸能而不是GABA能的。这些结果表明，由LHb引起的DA细胞的强大抑制作用不太可能来自VTA GABA神经元的选择性神经支配。此外，LHb可以介导DA细胞的直接激发，而DA细胞的直接激发被外源性来源的间接抑制所超越。

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