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首页> 外文期刊>The European Journal of Neuroscience >SPARC/osteonectin, an endogenous mechanism for targeting albumin to the blood-cerebrospinal fluid interface during brain development.
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SPARC/osteonectin, an endogenous mechanism for targeting albumin to the blood-cerebrospinal fluid interface during brain development.

机译：SPARC /骨粘连蛋白，一种在大脑发育过程中将白蛋白靶向血脑脊液界面的内源性机制。

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摘要

Specialized populations of choroid plexus epithelial cells have previously been shown to be responsible for the transfer of individual plasma proteins from blood to the cerebrospinal fluid (CSF), contributing to their characteristically high concentrations in CSF of the developing brain. The mechanism of this protein transfer remains elusive. Using a marsupial, Monodelphis domestica, we demonstrate that the albumin-binding protein SPARC (osteonectin/BM-40/culture-shock protein) is present in a subset of choroid plexus epithelial cells from its first appearance, throughout development, and into adulthood. The synthesis of SPARC by the lateral ventricular plexus was confirmed with real-time PCR. The expression level of SPARC was higher in plexuses of younger than older animals. Western blot analysis of the gene product confirmed the quantitative PCR results. The co-localization of SPARC and albumin shown by immunocytochemistry and its cellular location indicate that this glycoprotein may act as a recognition site for albumin. In addition, the numbers of SPARC-immunopositive cells and its expression were responsive to experimental changes of albumin concentration in the blood. It is suggested that SPARC may be one of the molecules that govern the uptake and delivery of proteins from blood to the CSF. The results also confirm that protein transfer across the blood-CSF barrier is developmentally and physiologically regulated.
机译：以前已显示脉络丛上皮细胞的专门种群负责将单个血浆蛋白从血液转移到脑脊液（CSF），这有助于它们在发育中的脑CSF中特征性地高浓度。这种蛋白质转移的机制仍然难以捉摸。使用有袋动物Monodelphis domestica，我们证明了白蛋白结合蛋白SPARC（骨粘连蛋白/ BM-40 /培养休克蛋白）从脉络丛上皮细胞的子集开始出现，发展到成年都存在。实时PCR证实了侧脑室丛的SPARC合成。在年轻的丛中，SPARC的表达水平高于年长的动物。基因产物的蛋白质印迹分析证实了定量PCR结果。免疫细胞化学显示SPARC和白蛋白的共定位及其细胞位置表明该糖蛋白可能充当白蛋白的识别位点。此外，SPARC免疫阳性细胞的数量及其表达对血液中白蛋白浓度的实验变化有反应。建议SPARC可能是控制蛋白质从血液向CSF摄取和传递的分子之一。该结果还证实了跨血CSF屏障的蛋白质转移在发育和生理上受到调节。

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