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首页> 外文期刊>The European Journal of Neuroscience >Proteolytic processing, axonal transport and differential distribution of chromogranins A and B, and secretogranin II (secretoneurin) in rat sciatic nerve and spinal cord.
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Proteolytic processing, axonal transport and differential distribution of chromogranins A and B, and secretogranin II (secretoneurin) in rat sciatic nerve and spinal cord.

机译：在大鼠坐骨神经和脊髓中，嗜铬粒蛋白A和嗜铬粒蛋白B的分泌蛋白水解过程，轴突运输和差异分布。

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摘要

The chromogranin family comprises chromogranin A and B, and secretogranin II. The present study has focused on the axonal transport of chromogranins/secretogranin II and their detailed distribution in peripheral nerves and the spinal cord. With radioimmunoassay (RIA) and column chromatography, we first studied the processing of chromogranin B and secretogranin II during axonal transport. No larger precursors of these peptides were detected in the sciatic nerves, indicating that they are already processed to a high degree early during axonal transport. We also analysed nerve segments above and below a crush, using RIA, in order to compare these accumulation data with those obtained by the cytofluorimetric-scanning (CFS) technique. For the latter technique, the amounts of accumulation distal to the crush (presumably representing recycling and retrogradely transported peptides) were 30-40% of the amounts in the proximal accumulation for chromogranin A and secretoneurin, in contrast to chromogranin B, which showed 15% recycling. With the RIA, the corresponding values for secretoneurin and PE-11 (antibody against chromogranin B) were 42% and 14%, respectively. Therefore, the data obtained by CFS were in excellent agreement with those obtained by RIA. In crushed sciatic nerves, chromogranin A was present in large axons as well as in small- and medium-sized axons. Chromogranin B was mainly restricted to large axons, while secretoneurin was localized to bundles of small axons. This differential distribution was also found in the spinal roots and in the peripheral terminals. Chromogranin A was present in both ventral and dorsal roots, and chromogranin B was detected in ventral roots and in large sensory axons in the dorsal roots. Secretoneurin was dominant in the dorsal root. Double-labelling studies with antibodies against choline acetyltransferase/vesicular acetylcholine transporter, or against tyrosine hydroxylase, confirmed that chromogranin A was distributed in cholinergic, sensory, as well as adrenergic neurons. Chromogranin B was mainly present in cholinergic motor neurons and large sensory neurons, and secretoneurin was restricted to adrenergic and sensory neurons. The present study demonstrates that chromogranins A and B, and secretoneurin are transported with fast axonal transport in the peripheral nerves, with different amounts of recycling, and that they are differentially distributed in different types of neurons in the peripheral nervous system and the spinal cord, suggesting that each of them may play a special role in subsets of neurons.
机译：嗜铬粒蛋白家族包含嗜铬粒蛋白A和B，以及促胰泌素II。目前的研究集中在嗜铬粒蛋白/分泌素II的轴突运输及其在周围神经和脊髓中的详细分布。通过放射免疫分析（RIA）和柱色谱，我们首先研究了轴突运输过程中嗜铬粒蛋白B和促胰液素II的加工过程。在坐骨神经中未检测到这些肽的较大前体，表明它们已经在轴突运输的早期被高度加工。我们还使用RIA分析了挤压物上方和下方的神经节段，以便将这些累积数据与通过细胞荧光扫描（CFS）技术获得的数据进行比较。对于后一种技术，压碎末梢的累积量（大概代表回收和逆向转运的肽）是嗜铬粒蛋白A和促分泌素的近端累积量的30-40％，而嗜铬粒蛋白B则为15％回收。对于RIA，促胰泌素尿素和PE-11（针对嗜铬粒蛋白B的抗体）的相应值分别为42％和14％。因此，CFS获得的数据与RIA获得的数据非常吻合。在破碎的坐骨神经中，嗜铬粒蛋白A存在于大型轴突以及中小型轴突中。嗜铬粒蛋白B主要限于大轴突，而促胰泌酸尿素则局限于小轴突束。在脊神经根和末梢也发现了这种差异分布。腹侧和背根均存在嗜铬粒蛋白A，腹侧根和背根中较大的感觉轴突均检测到嗜铬蛋白B。分泌神经苷在背根占主导。使用抗胆碱乙酰转移酶/囊泡乙酰胆碱转运蛋白或酪氨酸羟化酶抗体进行的双标记研究证实，嗜铬粒蛋白A分布在胆碱能，感觉神经和肾上腺素能神经元中。嗜铬粒蛋白B主要存在于胆碱能运动神经元和较大的感觉神经元中，而分泌神经尿素仅限于肾上腺素能和感觉神经元。本研究表明嗜铬粒蛋白A和B，以及促分泌素神经素以快速轴突运输方式在周围神经中运输，并具有不同程度的再循环，并且它们在周围神经系统和脊髓中的不同类型的神经元中分布不同，提示它们每个都可能在神经元子集中发挥特殊作用。

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