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首页> 外文期刊>The European Journal of Neuroscience >Immunohistochemical characterisation of Fos-positive cells in brainstem monoaminergic nuclei following intracranial self-stimulation of the medial forebrain bundle in the rat.
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Immunohistochemical characterisation of Fos-positive cells in brainstem monoaminergic nuclei following intracranial self-stimulation of the medial forebrain bundle in the rat.

机译：大鼠内侧前脑束的颅内自刺激后脑干单胺能核中Fos阳性细胞的免疫组织化学表征。

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摘要

Fos immunostaining was used as a marker of neuronal activity following intracranial self-stimulation (ICSS) of the medial forebrain bundle (MFB) in the rat, and was combined with immunostaining for tyrosine hydroxylase (TH), serotonin (5-HT), gamma-aminobutyric acid (GABA), or NR1 (one of the glutamate N-methyl- D-aspartate receptor subunits) for purposes of neurochemical identification. ICSS induced a significant but different degree of increase in the number of Fos-immunopositive (Fos+) cells in the six brainstem monoaminergic nuclei examined, which included the ventral tegmental area (VTA), substantia nigra pars compacta (SNc), dorsal raphe nucleus (DR), median raphe nucleus (MR), locus coeruleus (LC), and A7 noradrenaline cells. Densely labelled Fos+ cells were observed in the LC following ICSS, and many of these Fos+ cells were colocalized with TH. Similarly, many of Fos+ cells in the A7 and DR/MR were colocalized with TH and 5-HT, respectively. By contrast, a smaller number of Fos+ cells was detected in the VTA and SNc following the ICSS, and in these regions the majority of Fos+ cells were not colocalized with TH. Although results among regions quantitatively differed, the ICSS induced a significant increase in the number of double-labelled cells (GABA+/Fos+ or NR1+/Fos+) in all of the VTA, DR, and LC, in which the ICSS produced an ipsilaterally weighted increase in Fos-like immunoreactivity. These results suggest that ICSS of the MFB induces differential Fos expression within monoaminergic and GABAergic neurons in brainstem monoaminergic nuclei under modulation by glutamatergic afferents.
机译：Fos免疫染色被用作大鼠内侧前脑束（MFB）颅内自我刺激（ICSS）后神经元活性的标志物，并与酪氨酸羟化酶（TH），血清素（5-HT），γ的免疫染色相结合-氨基丁酸（GABA）或NR1（谷氨酸N-甲基-D-天冬氨酸受体亚基之一）用于神经化学鉴定。 ICSS在所检查的六个脑干单胺能核（包括腹侧被盖区（VTA），黑质致密部（SNc），背increase核（ DR），正中缝核（MR），蓝斑轨迹（LC）和A7去甲肾上腺素细胞。在ICSS之后的LC中观察到密集标记的Fos +细胞，并且其中许多Fos +细胞与TH共定位。同样，A7和DR / MR中的许多Fos +细胞分别与TH和5-HT共定位。相比之下，在ICSS之后，在VTA和SNc中检测到较少数量的Fos +细胞，在这些区域中，大多数Fos +细胞未与TH共定位。尽管区域间的结果在数量上有所不同，但ICSS导致所有VTA，DR和LC中双标记细胞（GABA + / Fos +或NR1 + / Fos +）的数量显着增加，其中ICSS导致同侧加权增加在类似Fos的免疫反应中。这些结果表明，MFB的ICSS在谷氨酸能传入受体的调节下诱导脑干单胺能核中单胺能和GABA能神经元内Fos表达的差异。

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