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首页> 外文期刊>The European Journal of Neuroscience >Subunits of the nitric oxide receptor, soluble guanylyl cyclase, expressed in rat brain.
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Subunits of the nitric oxide receptor, soluble guanylyl cyclase, expressed in rat brain.

机译：一氧化氮受体的亚基，可溶性鸟苷酸环化酶，在大鼠脑中表达。

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摘要

Despite the widespread use of nitric oxide as a signalling molecule in the central nervous system, the molecular makeup of its receptor, soluble guanylyl cyclase (sGC), therein is poorly understood. Accordingly, RT-PCR and in situ hybridization were used to identify sGC subunits expressed in rat brain. In addition to the expected mRNA for alpha 1 and beta1 subunits, message for the beta 2 subunit was detected in the cerebellum at all developmental stages investigated (1--150 days postnatum). The use of degenerate primers allowed the identification of mRNA coding for the rat alpha 2 subunit, which was also expressed at every age studied. All but beta 2 were detected by in situ hybridization in the brains of both 8-day-old and adult rats. The distribution patterns indicated that in some areas, e.g. caudate-putamen and nucleus accumbens, sGC probably exists mainly as the alpha 1 beta 1 heterodimer. In others, e.g. hippocampus and olfactory bulb, alpha 2 beta 1 is likely to be dominant. In the cerebellum, alpha 1 and beta 1 message was strong in the Purkinje cell layer but was not confined to Purkinje cells: smaller cells, presumed to be the Bergmann glia, were also labelled. In contrast, alpha 2 mRNA was concentrated in cerebellar granule cells. Western blotting indicated an excess of alpha 1 over beta 1 protein in the cerebellum, the reverse of what was found in the lung. It is concluded that, in molecular terms, sGC is likely to be more complex and exhibit more regional variation in the brain than previously thought. The functional consequences of this heterogeneity require investigation.
机译：尽管一氧化氮广泛用作中枢神经系统中的信号分子，但对其受体的可溶性鸟苷基环化酶（sGC）的分子组成知之甚少。因此，RT-PCR和原位杂交用于鉴定在大鼠脑中表达的sGC亚基。除了预期的α1和β1亚基的mRNA之外，在研究的所有发育阶段（产后1-150天），小脑中均检测到β2亚基的信息。使用简并引物可以鉴定出编码大鼠α2亚基的mRNA，该α2亚基也在每个研究的年龄都表达。除了β2以外，其他均在8日龄和成年大鼠的大脑中通过原位杂交检测到。分布模式表明在某些地区，例如尾状囊状核和伏伏核，sGC可能主要以α1β1异二聚体的形式存在。在其他情况下，例如在海马和嗅球中，alpha 2 beta 1可能占主导地位。在小脑中，Purkinje细胞层中的alpha 1和beta 1信息很强，但不仅限于Purkinje细胞：还标记了假定为Bergmann胶质细胞的较小细胞。相反，α2 mRNA集中在小脑颗粒细胞中。 Western印迹表明小脑中的alpha 1高于beta 1蛋白，这与在肺中发现的情况相反。结论是，就分子而言，sGC可能比以前认为的更为复杂，并且在大脑中表现出更多的区域差异。这种异质性的功能后果需要调查。

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